Abstract

With the improvement of living standards and frequently occurrence of food safety incidents, People pay more and more attention to food safety. Staphylococcus aureus is one of pathogens which could cause food spoilage. In this study, specifically primers has been designed and a rapid method has been established to detect S. aureus in food to shorten time of detection, increase the sensitivity of method and provide a technical help. PCR method has higher specificity, sensitivity, quickness and convenience, which may provide a new approach to detect SA in food quickly. Staphylococcus aureus was detected by PCR samples from food poisoning, in the case of the exclusion of other pathogens that can prompt this food poisoning is caused by Staphylococcus aureus. S. aureus enterotoxin is the main cause of food poisoning, which can be identified on its type enterotoxin produced under conditions permitting.

Keywords:

Food, rapid detection system, Staphylococcus aureus,

References

1. Atural News, 2008. MRSA Superbug Infections Now Killing More Americans than AIDS [C]. Citizen Journalist, March 10.
   
2. Boyce, J.M. and N.L. Havill, 2008. Comparison of BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains [J]. J. Clin. Microbiol., 46(1): 350-351.
   CrossRef    PMid:18032616 PMCid:PMC2224301    
3. Brooks, R.A., 1986. A robust layered control system for a mobile robot. IEEE J. Robot Autom., 2(1): 4-23.
   CrossRef    
4. Deurenberg, R.H. and E.E. Stobberingh, 2008. The evolution of Staphylococcus aureus [J]. Infect. Genet. Evol., 8(6): 747-763.
   CrossRef    PMid:18718557    
5. Feng, Y., C.J. Chen, L.H. Su, S. Hu, J. Yu and C.H. Chiu, 2008. Evolution and pathogenesis of Staphylococcus aureus: Lessons learned from genotyping and comparative genomics [J]. FEMS Microbiol. Rev., 32(1): 23-37.
   CrossRef    PMid:17983441    
6. Francois, P., S. Harbarth, A. Huyghe, G. Renzi, M. Bento, A. Gervaix, D. Pittet and J. Schrenzel, 2008. Methicillin-resistant Staphylococcus aureus, Geneva, Switzerland, 1993-2005 [J]. Emerg. Infect. Dis., 14(2): 304-307.
   CrossRef    PMid:18258126 PMCid:PMC2600191    
7. Kumar, S. and G.S. Mittal, 2010. Rapid detection of microorganisms using image processing parameters and neural network [J]. Food Bioprocess Tech., 3(5): 741-751.
   CrossRef    
8. Sievert, D.M., J.T. Rudrik, J.B. Patel, L.C. McDonald, M.J. Wilkins and J.C. Hageman, 2008. Vancomycin-resistant Staphylococcus aureus in the United States, 2002-2006. Clin. Infect. Dis., 46(5): 668-674.
   CrossRef    PMid:18257700    
9. Takano, T., K. Saito, L.J. Teng and T. Yamamoto, 2007. Spread of community-acquired Methicillin-Resistant Staphylococcus aureus (MRSA) in hospitals in Taipei, Taiwan in 2005, and comparison of its drug resistance with previous hospital-acquired MRSA [J]. Microbiol. Immunol., 51(6): 627-632.
   CrossRef    PMid:17579274    

Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.