Abstract

A new detection method for 1-naphthylacetic acid (NAA) based on the electrochemical immunosensor was introduced in the study, in which anti-NAA polyclonal antibody was used. An monolayer-molecular, self-reassemble membrane was made from 3-mercaptopropionic acid on the surface of gold electrodes, on the membrane the anti-NAA antibody was immoblize through ester-activated method with addition of 1-(3-Dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS). Based on the three-electrode system, an NAA immunosenory detection method was established with using the signal from cyclic voltammetry. The standard curve could be represented as △E = 0.0407lgc+0.0701(R² = 0.9984) with the range from 0.05 μg/mL to 2 μg/mL and with the detection limit was 0.035 μg/mL. The sensory method had very low cross-reactivity, lower than 3%, to NAA similarities such as phenylacetic acid and indolyl-3-acetic acid. In addition, the sensor could be used with high stability as long as 30 days (room temperature). It indicated that a specific, stable electrochemical immunosensory detection method for 1-Naphthaleneacetic acid was successfully developed.

Keywords:

1-naphthylacetic acid, electrochemical immunosensor, self-assembled monolayer membrance,

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