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     Advance Journal of Food Science and Technology


Evaluation of Antioxidative Properties of Various Extracts from Cyclina sinensis

1, 2Lei Guo, 2Yanli Liu and 2Chao Liu
1Jiangsu Institute of Marine Resources, Lianyungang 222004, P.R. China
2School of Ocean, Huaihai Institute of Technology, Lianyungang 222005, P.R. China
Advance Journal of Food Science and Technology  2014  4:558-562
http://dx.doi.org/10.19026/ajfst.6.73  |  © The Author(s) 2014
Received: January 20, 2014  |  Accepted: January 30, 2014  |  Published: April 10, 2014

Abstract

The mollusk Cyclina sinensis has been widely used as a food and folk medicine in China, Korea and south eastern Asia. In this study, Total Water-soluble Extracts (TWE), Crude Polysaccharides (CPS), Total Crude Proteins (TCP) were isolated from C. sinensis and the Deproteinized Polysaccharides (DPS) was obtained after deproteination of CPS. Their contents, such as neutral carbohydrate, protein and uronic acid, were measured. Their antioxidative properties against 1, 1'-Diphenyl-2-Picrylhydrazyl (DPPH) free radical, hydroxyl free radical and superoxide anion free radical, as well as their reducing capacity were investigated. Results showed that various fractions of C. sinensis can scavenge all free radicals tested above and possessed the increasing reducing capacity in a dose-dependent mode. Compared with the other three fractions, TWE represents the comprehensively best antioxidative properties. The antioxidative activities of TWE from C. sinensis might contribute to antioxidant-related functional food and pharmaceutical industries.

Keywords:

Antioxidative activity, Cyclina sinensis, polysaccharide, protein, total water-soluble extracts,


References

  1. Blumenkrantz, N. and G. Asboe-Hansen, 1973. New method for quantitative determination of uronic acids. Anal. Biochem., 54(2): 484-489.
    CrossRef    
  2. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72(1-2): 248-254.
    CrossRef    PMid:942051    
  3. Chen, Y., M.Y. Xie, S.P. Nie, C. Li and Y.X. Wang, 2008. Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chem., 107(1): 231-241.
    CrossRef    
  4. Duan, J. and D.L. Kasper, 2011. Oxidative depolymerization of polysaccharides by reactive oxygen/nitrogen species. Glycobiology, 21(4): 401-409.
    CrossRef    PMid:21030538 PMCid:PMC3055593    
  5. Gu, R.R., Y.S. Yu and Y.L. Cai, 2006. Analysis and evaluation of the nutritive composition of Cyclina sinensis. Chin. J. Zool., 41(3): 70-74.
  6. Guo, L. and C. Liu, 2012. Extraction and antioxidant activity of ultrasonic-assisted flavonoids from Suaeda salsa. Appl. Mech. Mater., 140: 343-349.
    CrossRef    
  7. Guo, L., M.S. Lv, S.J. Wang, B. Lu and X.L. Lu, 2010. Determination of total sugar from cherry wine by phenol-sulfuric acid method. Food Res. Dev., 31(6): 130-132.
  8. Guo, L., W.C. Zhu, Y.T. Liu, J.Y. Wu, A.Q. Zheng and Y.L. Liu, 2013. Response surface optimized extraction of flavonoids from mimenghua and its antioxidant activities In vitro. Food Sci. Biotechnol., 22(5): 1285-1292.
    CrossRef    
  9. Huang, W., A. Xue, H. Niu, Z. Jia and J. Wang, 2009. Optimised ultrasonic-assisted extraction of flavonoids from Folium eucommiae and evaluation of antioxidant activity in multi-test systems in vitro. Food Chem., 114(3): 1147-1154.
    CrossRef    
  10. Jiang, C., M. Wang, J. Liu, D. Gan and X. Zeng, 2011. Extraction, preliminary characterization, antioxidant and anticancer activities in vitro of polysaccharides from Cyclina sinensis. Carbohyd. Polym., 84(3): 851-857.
    CrossRef    
  11. Kanatt, S.R., R. Chander and A. Sharma, 2007. Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chem., 100(2): 451-458.
    CrossRef    
  12. Li, X.Y., Z.G. Dong, B.L. Yan, H.L. Cheng, X.P. Meng, H.D. Shen and J.L. Li, 2010. Analysis and evaluation of nutritional components in Cyclina sinensis and Meretrix meretrix. Food Sci., 31(23): 366-370.
  13. Liu, W.S., Y.H. Ma, S.Y. Hu, G.H. Miao and J.H. Li, 2002. Rearing Venus clam seeds, Cyclina sinensis (Gmelin), on a commercial scale. Aquaculture, 211(1-4): 109-114.
    CrossRef    
  14. Nagai, T., R. Inoue, H. Inoue and N. Suzuki, 2003. Preparation and antioxidant properties of water extract of propolis. Food Chem., 80(1): 29-33.
    CrossRef    
  15. Podsedek, A., 2007. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Sci. Technol., 40(1): 1-11.
    CrossRef    
  16. Qi, H., Q. Zhang, T. Zhao, R. Hu, K. Zhang and Z. Li., 2006. In vitro antioxidant activity of acetylated and benzoylated derivatives of polysaccharide extracted from Ulva pertusa (Chlorophyta). Bioorg. Med. Chem. Lett., 16(9): 2441-2445.
    CrossRef    PMid:16481163    
  17. Reiter, R.J., D. Melchiorri, E. Sewerynek, B. Poeggeler, L. Barlow-Walden, J. Chuang, G.G. Ortiz and D. Acu-a-Castroviejo, 1995. A review of the evidence supporting melatonin's role as an antioxidant. J. Pineal Res., 18(1): 1-11.
    CrossRef    PMid:7776173    
  18. Smirnof, N. and Q. Cumbes, 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry, 28(4): 1057-1060.
    CrossRef    
  19. Turkoglu, A., M.E. Duru, N. Mercan, I. Kivrak and K. Gezer, 2007. Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chem., 101(1): 267-273.
    CrossRef    
  20. Wang, X.Q., M. Cao, B.L. Yan, S. Ma and S.L. Dong, 2006. Biology and reproduction of Cyclina sinensis. Fisheries Sci., 25(6): 312-316.
  21. Wang, L.P., R.Q. Geng, Y. Liu, H.B. Zhang, Y. Chen and F. Xue, 2007. Status of research and utilization of Cyclina sinensis germplasm resources in China. Jiangsu Agr. Sci., 4: 254-255.
  22. Yu, L., S. Haley, J. Perret, M. Harris, J. Willson and M. Qian, 2002. Free radical scavenging properties of wheat extracts. J. Agr. Food Chem., 50(6): 1619-1624.
    CrossRef    PMid:11879046    
  23. Zhang, G., L. He and M. Hu, 2011. Optimized ultrasonic-assisted extraction of flavonoids from Prunella vulgaris L. and evaluation of antioxidant activities in vitro. Innov. Food Sci. Emerg., 12(1): 18-25.
    CrossRef    

Competing interests

The authors have no competing interests.

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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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