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Abstract
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Article Information:
Identification and Characterization of FaFT1: A Homolog of FLOWERING LOCUS T from Strawberry
Hengjiu Lei, Xiao Guo, Yantao Wang, Liping Yao, Shuang Wang and Tianhong Li
Corresponding Author: Tianhong Li
Submitted: December 4, 2014
Accepted: January 8, 2015
Published: May 15, 2015 |
Abstract:
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FLOWERING LOCUS T(FT) -like genes play crucial roles in flowering transition in several plant species. In this study, a homolog of FT, designated as FaFT1, was isolated and characterized from strawberry. The open reading frame of FaFT1 was 531 bp, encoding a protein of 176 amino acids. Phylogenetic and sequence analysis showed that the FaFT1 protein contained the conservation of Tyr84 and Gln139, as well as the highly conserved amino acid sequences LGRQTVYAPGWRQN and LYN and that it was a member of the FT-like genes of dicots. Subcellular localization analysis revealed that the FaFT1 protein mainly localized in the nuclei of the Arabidopsis protoplasts. FaFT1 was highly expressed in strawberry mature leaves and its expression level decreased under floral induction conditions. Additionally, FaFT1 expression exhibited diurnal circadian rhythm both under SD and LD conditions. Over expression of FaFT1 in wild-type Arabidopsis caused early flowering. Taken together, these results indicate that FaFT1 is a putative FT homolog in strawberry, acting as a floral promoter in Arabidopsis
Key words: Arabidopsis, FaFT1, flowering, strawberry, , ,
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Cite this Reference:
Hengjiu Lei, Xiao Guo, Yantao Wang, Liping Yao, Shuang Wang and Tianhong Li, . Identification and Characterization of FaFT1: A Homolog of FLOWERING LOCUS T from Strawberry. Advance Journal of Food Science and Technology, (3): 180-188.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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