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     Advance Journal of Food Science and Technology

    Abstract
2015(Vol.7, Issue:6)
Article Information:

Antioxidant Activity of Peptides from Fermented Milk with Mix Culture of Lactic Acid Bacteria and Yeast

Yun Li, Tongjie Liu and Guoqing He
Corresponding Author:  Guoqing He 
Submitted: ‎September ‎22, ‎2014
Accepted: October ‎24, ‎2014
Published: February 25, 2015
Abstract:
The aim of the present study is to investigate the production of antioxidant peptides during milk fermentation with co-culture of Lactic Acid Bacteria (LAB) and yeast. Five LAB strains, previously screened with higher hydrolysis activity and Debaryomyces hansenii H2 which isolated from Tibet kefir were used in the study. The peptides separated from fermented milk were analysed antioxidant activity with DPPH radical scavenging, hydroxyl radical scavenging, chelation of metal ions and reducing power assays. The growth of Streptococcus. thermophilus Lactobacillus. delbrueckii ssp. bulgaricus and Lactococcus. lactis was enhanced with co-cultures and L. acidophilus was inhibited in co-culture with yeast. In co-culture with yeast, a significant decrease of the acidity was observed among all the fermentation and the pH reached higher values than in single LAB cultures. Except for L.delbrueckii ssp. bulgaricus, there was no significant difference of protein hydrolysis with other test LAB strains between co-culture and single culture. The co-incubation of LAB with the yeast developed a stronger antioxidant activity in DPPH radical and hydroxyl radical scavenging and no significant (p>0.05) difference in chelation of metal ions. The reducing power of L.delbrueckii ssp. bulgaricus and L. helveticus in co-culture was significant higher than those of single culture.

Key words:  Antioxidant peptides, co-culutre, Debaryomyces hansenii, fermented milk, lactic acid bacteria, ,
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Cite this Reference:
Yun Li, Tongjie Liu and Guoqing He, . Antioxidant Activity of Peptides from Fermented Milk with Mix Culture of Lactic Acid Bacteria and Yeast. Advance Journal of Food Science and Technology, (6): 422-427.
ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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