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2013 (Vol. 5, Issue: 07)
Article Information:

Research on Extraction and Characterization of Cellulase from Commercial Enzyme Preparation

Guowei Shu, Hui Yang, He Chen, Zhiwei Yang
Corresponding Author:  Guowei Shu 

Key words:  Carboxymethylcellulase, cellulase, filter paper activity, enzymatic property, salting out, ,
Vol. 5 , (07): 839-842
Submitted Accepted Published
February 15, 2013 March 13, 2013 July 05, 2013
Abstract:

The extraction of cellulase from commercial enzyme preparation by ammonium sulfate precipitation and its enzymatic characterization were studied. The results were as follows: the conditions of precipitation was 60% saturation of ammonium sulfate, the recovery rate of carboxymethylcellulase (CMCase) and Filter Paper enzyme Activity (FPA) were 73.8 and 71.4%, respectively. The enzyme has optimal temperature of 55C, optimal pH of 4.8, the ions of Co2+, Mn2+ in the buffer lowered the activity of CMCase, but the Cu2+ in low concentration activated the CMCase. The ions of Co2+, Mn2+ and Al2+ in the buffer lowered the activity of FPA, but the Cu2+ and K+ in low concentration activated the FPA; Km and Vmax of the enzyme were 2.2210-3 g/mL and 1.11104 U/h using Lineweaver-Burk graphic method, respectively.
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  Cite this Reference:
Guowei Shu, Hui Yang, He Chen, Zhiwei Yang, 2013. Research on Extraction and Characterization of Cellulase from Commercial Enzyme Preparation.  Advance Journal of Food Science and Technology, 5(07): 839-842.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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