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2013 (Vol. 5, Issue: 06)
Article Information:

Culture Conditions affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase

Jiazhou Li, Yuanyuan Zhang, Mingquan Xie and Xiaochun Luo
Corresponding Author:  Xiaochun Luo 

Key words:  Decaprenyl diphosphate synthase, Escherichia coli, Rhizobium radiobacter, ubiquinone, , ,
Vol. 5 , (06): 732-737
Submitted Accepted Published
January 31, 2013 February 25, 2013 June 05, 2013
Abstract:

Ubiquinones (UQ) are important electron transporters and play lot of important roles in most organisms. In different species, UQ was classified to be UQ-6, 7, 8, 9, 10 according to their polyprenyl side chain length. The side chain’s length is determined by the enzyme named Poly-Prenyl diphosphate synthases (PPPS). Bacteria are usually reconstructed to producing UQ-10 used in human’s food additive, medicine or cosmetics, such as using decaprenyl Diphosphate Synthase (DPS) gene from R. radiobacter to substitute E.coli’s octaprenyl diphosphate synthase gene , just like E. coli BL21 (△ispB::ddsA) used in this study. It is interesting that not only in these reconstructed bacteria, but in human-being, DPS can synthesize UQ-9 besides UQ-10. The mechanism of this phenomenon is still unknown. In this study, the effects of culture conditions, including the temperature, dissolved oxygen, pH and culture medium, on the DPS characteristics in E. coli BL21 (△ispB::ddsA) were examined. Results show that temperature greatly affects the ratio of UQ-9/UQ-10, but not the total ubiquinone’s production. Increasing dissolved oxygen and protein concentration in culture medium can promote total ubiquinone’s production, but not the ratio of UQ-9/UQ-10. These results may give reference for UQ-10’s industrial produce and the mechanism of these conditions’ effect on DPS will be discussed.
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  Cite this Reference:
Jiazhou Li, Yuanyuan Zhang, Mingquan Xie and Xiaochun Luo, 2013. Culture Conditions affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase.  Advance Journal of Food Science and Technology, 5(06): 732-737.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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