Home           Contact us           FAQs           
 
   Journal Page   |   Aims & Scope   |   Author Guideline   |   Editorial Board   |   Search
    Abstract
2012 (Vol. 4, Issue: 5)
Article Information:

Cloning of Lactate dehydrogenase Gene and Effect on the Waterlogging Tolerance of Brassica napus L.

Ben-bo Xu, Ling-li Xie, Yong Cheng, Guang-yuan Lu, Pu-ying Zheng and Xue-kun Zhang
Corresponding Author:  Ben-bo Xu 

Key words:  Brassica napus L., cloning, lactate dehydrogenase, waterlogging tolerance, waterlogging tolerance, index lipase,
Vol. 4 , (5): 332-336
Submitted Accepted Published
August 31, 2012 October 03, 2012 October 20, 2012
Abstract:

To investigate the mechanism on waterlogging tolerance in Brassica napus, 12 B. napus cultivars with different waterlogging tolerance were used in the research and Waterlogging Tolerance Index (WTI) was calculated by multiplying relative percentage germination and the relative seedling height. The results indicated that Lactate Dehydrogenase (LDH) enzyme activity rapidly increased at 24 h after waterlogging treatment and reached peak between 48-72 h. WTI was correlated with LDH enzyme activity at 24 h after water logging treatment and the correlation coefficient between them was 0.84. Transcription level of the BnLDH had significant difference in the 12 lines after waterlogging treatment. BnLDH expression level was very low before waterlogging treatment and induced by waterlogging treatment and arrived at peak at 48 h. Correlation analysis indicated correlation coefficient between WTI and BnLDH expression at 24 and 48 h after waterlogging treatment was 0.56 and 0.72, respectively. An LDH gene, denoted BnLDH-1, was cloned from oilseeds by the Rapid Amplification of CDNA Ends (RACE) from 12 materials and the results indicated all of them had same protein sequence.
Abstract PDF HTML
  Cite this Reference:
Ben-bo Xu, Ling-li Xie, Yong Cheng, Guang-yuan Lu, Pu-ying Zheng and Xue-kun Zhang, 2012. Cloning of Lactate dehydrogenase Gene and Effect on the Waterlogging Tolerance of Brassica napus L..  Advance Journal of Food Science and Technology, 4(5): 332-336.
    Advertise with us
 
ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
Submit Manuscript
   Current Information
   Sales & Services
   Contact Information
  Executive Managing Editor
  Email: admin@maxwellsci.com
  Publishing Editor
  Email: support@maxwellsci.com
  Account Manager
  Email: faisalm@maxwellsci.com
  Journal Editor
  Email: admin@maxwellsci.com
  Press Department
  Email: press@maxwellsci.com
Home  |  Contact us  |  About us  |  Privacy Policy
Copyright © 2009. MAXWELL Science Publication, a division of MAXWELLl Scientific Organization. All rights reserved