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    Abstract
2011 (Vol. 3, Issue: 6)
Article Information:

Determination of Yeasts Antimicrobial Activity in Milk and Meat Products

L.B. Roostita, G.H. Fleet, S.P. Wendry, Z.M. Apon and L.U. Gemilang
Corresponding Author:  L.B. Roostita 

Key words:  Antimicrobial activity, milk and meat products, yeasts, , , ,
Vol. 3 , (6): 442-445
Submitted Accepted Published
2011 October, 31 2011 November, 18 2011 December, 25
Abstract:

The research was arranged to isolate yeasts from livestock products and then the yeasts antimicrobial activity was tested towards putrefaction and pathogenic bacteria. Yeasts isolated from livestock products using Malt Extract Agar (MEA), the total yeasts population counted with using total plate count method, antimicrobial activity tested using diffusion methods against Pseudomonas aerugenes, Staphylococcus aureus and Escherichia coli and then the chosen isolate identified with using 18s RNA method. The results have shown that the total yeasts population on pasteurized cow’s milk were 1.2×106 cfu/g, fruit yoghurt 5.4×106 cfu/g, lamb meat 1×105 cfu/g, beef 1×105 cfu/g and beef sausages 1×106 cfu/g total yeasts population. Fruit yoghurt isolate shown the best antimicrobial activity with 35 mm clear zone diameter against Pseudomonas aerugenes, 8 mm clear zone diameter against Staphylococcus aureus and 10 mm clear zone diameter against Escherichia coli. The 18 s RNA test shown that fruit yoghurt isolate was 100% (FR3-F primer) and 99% (FR3-R primer) identical with Candida parapsilosis.
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  Cite this Reference:
L.B. Roostita, G.H. Fleet, S.P. Wendry, Z.M. Apon and L.U. Gemilang, 2011. Determination of Yeasts Antimicrobial Activity in Milk and Meat Products.  Advance Journal of Food Science and Technology, 3(6): 442-445.
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