Research Article | OPEN ACCESS
Improving Salt Tolerance in Tomato Lycopersicon esculentum Mill. by in Vitro Mutation Technique
Zahra N. Al Hattab, Ekhlas A. El Kaaby, Wajida Kh. Al Qaudhy, Jenan A. Al Ani and Ashwaq Abdul Razaq
Department of Genetic Engineering, Directorate of Agriculture Research, Ministry of Science and Technology, P.O Box 765, Baghdad, Iraq
Current Research Journal of Biological Sciences 2016 3:24-27
Received: September ‎30, ‎2015 | Accepted: January ‎2, ‎2016 | Published: July 20, 2016
Abstract
This research was conducted to produce salt tolerant tomato plants by using mutation and tissue culture techniques. Seeds of two Tomato cultivars (Super Regina and Trescantos) were treated with 2.0 mM of Sodium Azide NaN3 for 4 h and sterilized with Sodium Hypochlorite (NaOCl) of (0.0, 1.0 or 3.0)%. The treated seeds were germinated on (MS) medium and shoot tips (1 cm) were used to induce calli on media supplemented with different hormones combinations. The produced calli were cultured on media of (6.5, 8.5, or 10.5) dSm-1 sodium chloride. Plants were then regenerated from the survival calli. The results showed that 3% NaOCl was the best sterilization treatment. Moreover the best growth regulator combinations were either 2 mg /L BA with 2 mg/L IAA or 0.5 mg of BA with 1 mg/L IAA. Since there was no significant difference between them, the last was recommended. Furthermore, salinity had negative effect on callus growth and (6.5 dSm-1) treatment showed significantly higher callus fresh and dry weights than the other treatments. No plants were produced from 10.5 dSm-1 treatment and Super Regina showed higher percentage of plant regeneration than Trescantos. Calli grown on 10.5 dSm-1 contained the highest Sodium and Chloride ions. Trescantos cultivar showed significantly higher calli sodium ions content compared with Super Regina. However, no significant differences between the two cultivars in the Chloride ion content were found. In conclusion it has been possible to induce and select salt tolerant tomato plants via in vitro mutation technique.
Keywords:
BA, IAA, shoot tip, sodium azide, sodium chloride,
Competing interests
The authors have no competing interests.
Open Access Policy
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Copyright
The authors have no competing interests.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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