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     Current Research Journal of Biological Sciences


Genomic Island Location in Acinetobacter baumannii Strains by tRIP-PCR Technique

Suhadsaad, Alice K. Melconian, Ali H. Ad'hiah and Kumar Rajakumar
Biotechnology Department, College of Science, University of Baghdad, Iraq
Current Research Journal of Biological Sciences  2013  6:296-301
http://dx.doi.org/10.19026/crjbs.5.5432  |  © The Author(s) 2013
Received: August 01, 2013  |  Accepted: August 26, 2013  |  Published: November 20, 2013

Abstract

This study was performed to detect the presence of genomic islands which usually insert in the tRNA genes and other non-coding RNA genes, in this study eight strains of Acinetobacter baumannii (AYE, A457, A14, A424 A473, A92, ACICU, A25) were tested by used of tRIP-(tRNA site Interrogation for Pathogeni city islands, prophases and other GIs)-PCR method. The results of PCR and agarose gel electrophoresis for eight strains of two loci #7, #24 were: the results of #7 loci screening showed that all strains were positive except A. Baumannii A457 strain was negative. While the results of #24 loci showed presence of foreign DNA in A. Baumannii AYE, A457, A14, A424, A473, A92 except the results of (ACICU, A25) was positive.

Keywords:

Acinetobacter baumannii, genomic islands, tRIP-PCR,


References


Competing interests

The authors have no competing interests.

Open Access Policy

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Copyright

The authors have no competing interests.

ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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