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Abstract
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Article Information:
Response of Nine Cultivars of Pepper (Capsicum spp.) to Infection by Four Viruses under Natural Field Conditions in the Coastal Savanna Zone of Ghana
A.S. Appiah, E.K. Quartey, H.M. Amoatey, W. Nunekpeku, M. Owusu-Ansah and S. Ofori, Biotechnology Center
Corresponding Author: Andrew Sarkodie Appiah
Submitted: October 09, 2012
Accepted: December 03, 2012
Published: February 05, 2014 |
Abstract:
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A study was conducted to evaluate the reaction of two local and seven exotic pepper cultivars to four viral diseases in the coastal savanna zone of Ghana. The objective was to evaluate the response of the pepper cultivars to the viruses under natural field conditions. The plants were assessed for symptom severity as well as disease incidence. Symptom expression varied among the cultivars, with severity ranging from 0.5% at 4 Weeks after Transplanting (WAT) to 47.2% at 14 WAT. Disease incidence also varied significantly among the cultivars and ranged from 1.1% at 4 WAT to 61.3% at 14 WAT. Enzyme-Linked Immune Sorbent Assay (ELISA) was performed against PVMV, TMV, CMV and PMMV for each cultivar. The test showed the presence of all four viruses in the leaf extracts, with each cultivar infected by at least one of the four viruses. PVMV and CMV were the predominant viruses identified. Mixed infections of two or more viruses were observed in all the cultivars. TMV was detected in all cultivars except the two hybrids, Sunny F1 and Forever F1, while PMMV was detected in only cv. Legon 18 which had a mixed infection of all four viruses.
Key words: Capsicum spp.,, cultivar, disease incidence, enzyme-linked immunosorbent assay, mixed infection, symptom severity, viruses
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Cite this Reference:
A.S. Appiah, E.K. Quartey, H.M. Amoatey, W. Nunekpeku, M. Owusu-Ansah and S. Ofori, Biotechnology Center, . Response of Nine Cultivars of Pepper (Capsicum spp.) to Infection by Four Viruses under Natural Field Conditions in the Coastal Savanna Zone of Ghana. Research Journal of Applied Sciences, Engineering and Technology, (5): 903-907.
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ISSN (Online): 2040-7467
ISSN (Print): 2040-7459 |
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