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2013 (Vol. 5, Issue: 23)
Article Information:

Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology

Guowei Shu, Hui Yang, He Chen and Shikai Wang
Corresponding Author:  Guowei Shu 

Key words:  Cellulose, carboxymethyl cellulose, FPA, response surface methodology, solid state fermentation, ,
Vol. 5 , (23): 5438-5442
Submitted Accepted Published
November 24, 2012 January 17, 2013 May 28, 2013
Abstract:

Response Surface Methodology (RSM) based on a three-level, three-variable Box and Behnken Factorial Design (BBFD) was used to evaluate the interactive effects of corn stalk: bran ratio, tween80 and temperature on the cellulase production by solid fermentation. The optimum conditions derived via RSM were: corn stalk: bran ratio 1.29:1, Tween80 11.05 &muL and temperature 31 C for carboxymethyl cellulase (CMCase) and corn stalk: bran ratio 0.77:1, Tween80 12.54&muL and temperature 32 C for Filter Paper Activity (FPA). The actual experimental yield was 406.42 U/g for CMCase and 93.62 U/g for FPA under optimum condition, which compared well to the maximum predicted value of 405.67 U/g and 91.29 U/g. The cellulase yield under optimal conditions was 1.45 fold for CMCase and 1.33 fold for FPA to the control.
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  Cite this Reference:
Guowei Shu, Hui Yang, He Chen and Shikai Wang, 2013. Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology.  Research Journal of Applied Sciences, Engineering and Technology, 5(23): 5438-5442.
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ISSN (Online):  2040-7467
ISSN (Print):   2040-7459
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