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2011 (Vol. 3, Issue: 1)
Article Information:

In ovo Administration of Ghrelin and Subsequent Intestinal Leucine aminopeptidase (LAP) Activity in Broiler Chickens

J. Ghiasi Ghaleh-kandi, A.R. Lotfi, R. Beheshti, H. Aghdam Shahryar, H. Karamouz and K. Nazeradl
Corresponding Author:  Alireza Lotfi 

Key words:  Chicken, ghrelin, In ovo injection, intestine, Leucine Aminopeptidase (LAP), ,
Vol. 3 , (1): 14-17
Submitted Accepted Published
2010 October, 14 2010 November, 05 2011 February, 05
Abstract:

Aim of this study was to investigation on effect of in ovo administration of ghrelin on subsequent Leucine Aminopeptidase (LAP) activity in broiler chickens. In this experiment 250 fertilized eggs were collected from commercial breeder flock. The eggs were divided into five experimental groups; control T1 (without injection), group T2 (in ovo injected with solution), group T3 (in ovo injected with 50 μg/egg ghrelin), group T4 (in ovo injected with 100 μg/egg ghrelin) and group T5 (in ovo injected with 150 μg/egg ghrelin). All of groups were incubated. In ovo injection was done at day 7 of incub ation. in ovo administration of 150 μg/egg ghrelin in embryonic period, could stimulate LAP activity at 21-day- old chicks in 10, 30 and 50% of intestine with 3520.4, 266.9, 4595.6 IU/g protein, also in ovo injected 50 and 150 μg/egg ghrelin could stimulate LAP activity in 1, 50 and 70% of intestine with 3071.4, 4779.3 and 5013.4 IU/g. In 42-day-old chicks, in ovo injected 50 μg/egg ghrelin could stimulate LAP activity in 1, 10, 30, 40, 70, and 90% percent of intestine. These findings demonstrated stimulatory effects of ghrelin in low doses (50 μg) in chicken intestine LAP activity.
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  Cite this Reference:
J. Ghiasi Ghaleh-kandi, A.R. Lotfi, R. Beheshti, H. Aghdam Shahryar, H. Karamouz and K. Nazeradl, 2011. In ovo Administration of Ghrelin and Subsequent Intestinal Leucine aminopeptidase (LAP) Activity in Broiler Chickens.  International Journal of Animal and Veterinary Advances, 3(1): 14-17.
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