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Abstract
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Article Information:
Evaluation of the Antimicrobial Activity of the K9CATH Peptide (38 Amino Acids) Against a Mastitis Isolated Strain of Staphylococcus aureus by the Resazurin microtiter Method
Albero Barreras-Serrano, Alma Rossana Tamayo-Sosa, Víctor Manuel del Villar-Perez, Alma Angelina Castellanos-Félix, Luis Tinoco-Gracia and Tonatiuh Melgarejo
Corresponding Author: Alma Rossana Tamayo-Sosa
Submitted: December 28, 2012
Accepted: January 17, 2013
Published: April 20, 2014 |
Abstract:
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The antimicrobial activity of the synthetic peptide K9CATH was determined by the Resazurin microtitre Method (RMM) against a strain of S. aureus isolated from a case of mastitis. To the antibiogram this bacteria strain showed to be resistant to Ampicillin, Erythromycin, Cefeprime, Dicloxaciline and Penicillin (10 U), while the MIC obtained for the K9CATH was 5.66 &mug/mL. Unlike the reference broth method, visual reading for MIC determination with the RMM showed to be easier, rapid, inexpensive and more sensitive for antimicrobial peptide screening, based in a color change from blue (not growth) to pink (growth). This is the first time that the resazurin method is used to determine the MIC of the 38 aa´s K9CATH peptide against a mastitic isolate of S. aureus.
Key words: AMPs and mastitis, K9CATH peptide, resazurin assay, Staphylococcus aureus, , ,
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Cite this Reference:
Albero Barreras-Serrano, Alma Rossana Tamayo-Sosa, Víctor Manuel del Villar-Perez, Alma Angelina Castellanos-Félix, Luis Tinoco-Gracia and Tonatiuh Melgarejo, . Evaluation of the Antimicrobial Activity of the K9CATH Peptide (38 Amino Acids) Against a Mastitis Isolated Strain of Staphylococcus aureus by the Resazurin microtiter Method. International Journal of Animal and Veterinary Advances, (2): 58-60.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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