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     International Journal of Animal and Veterinary Advances

    Abstract
2013(Vol.5, Issue:4)
Article Information:

Negative Prediction Value of Pregnancy-associated Glycoprotein Contributes to Reduce the Days During Which Nonpregnant Holstein Cows are Subjected to Diverse Strategies of Hormonal Synchronization

Ilda G. Fernández, José Moncebáez, Carlos Elizondo, Horacio Hernández, Raúl Ulloa-Arvizu and Susana Rojas
Corresponding Author:  Ilda G. Fernández 
Submitted: March 01, 2013
Accepted: March 27, 2013
Published: August 20, 2013
Abstract:
The objective of the present study was to determine the accuracy and the negative prediction value of Pregnancy-Associated Glycoprotein (PAG) at 29 d post artificial insemination by ELISA. Blood serums of multiparous Holstein cows were used (n = 275). Pregnancy diagnosis was carried out by rectal palpation at 40 d and it was considered as reference test. Pregnancy results based on the PAG-ELISA and the pregnancy diagnosis at 40 d showed high concordance with a kappa value of 0.7. The accuracy test based on PAG-ELISA at 29 d post artificial insemination compared with pregnancy diagnosis by rectal palpation was 88%. The values of sensibility, specificity, positive prediction value, negative prediction value and positive and negative likelihood ratio were 96, 78, 83 and 95%, 4.5 and 0.0442, respectively. It is concluded that pregnancy diagnosis of PAG-ELISA is reliable and accurate at 29 d post artificial insemination. The PAG-ELISA's negative prediction value diminishes the number of days in subjecting cows diagnosed as nonpregnant to diverse strategies of hormonal synchronization protocols.

Key words:  Dairy cattle, pregnancy diagnosis, pregnancy-associated glycoprotein, rectal palpation, , ,
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Cite this Reference:
Ilda G. Fernández, José Moncebáez, Carlos Elizondo, Horacio Hernández, Raúl Ulloa-Arvizu and Susana Rojas, . Negative Prediction Value of Pregnancy-associated Glycoprotein Contributes to Reduce the Days During Which Nonpregnant Holstein Cows are Subjected to Diverse Strategies of Hormonal Synchronization. International Journal of Animal and Veterinary Advances, (4): 160-164.
ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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