Home           Contact us           FAQs           
   Journal Page   |   Aims & Scope   |   Author Guideline   |   Editorial Board   |   Search
2012 (Vol. 4, Issue: 6)
Article Information:

Evidence of Avian Leukosis Virus Subgroup E and Endogenous Avian Virus in Marek’s Disease Vaccines Derived from Chicken Embryo Fibroblasts

N.R. Dhanutha, M.R. Reddy and S.S. Lakshman Rao
Corresponding Author:  M.R. Reddy 

Key words:  ALV-E, avian leucosis virus, chicken, env gene, ev loci, marek’s disease vaccine, sequencing,
Vol. 4 , (6): 363-369
Submitted Accepted Published
August 25, 2012 September 24, 2012 December 20, 2012

The aim of this study was to detect and characterize the endogenous ALVs in cell associated MD vaccine. Chicken embryo fibroblast cell associated Marek’s disease vaccine was tested for possible contamination with Avian Leukosis Viruses (ALVs). Initially the vaccine cell lysate was tested for presence of group specific antigen (p27) of ALVs by ELISA and found positive for GSA. Subsequently total DNA and RNA was isolated from vaccine CEFs and analyzed by PCR and RT-PCR using primers specific for ALV subgroups A-E and J. Subgroup specific PCR and RT-PCR revealed that the CEFs were positive for ALV-E and negative for all other exogenous ALV subgroups (ALV-A, B, C, D and J). Envelope gp85 gene sequence alignment and phylogenetic analysis further confirmed that the ALV sequences found in CEFs of MD vaccine were belongs to endogenous ALV-E. Further this sequence has high homology with endogenous loci ev-1, ev-3 and ev-6. Amplification of genomic DNA with endogenous virus locus specific primers revealed that the CEFs of MD vaccine possess ev-1 and ev-6 and negative for ev-3, ev-9 and ev-21. In conclusion, the data in this study clearly demonstrated that the cell associated commercial MD vaccine tested was contaminated with an endogenous subgroup E and also possess ev-loci such as ev1 and ev-6.
Abstract PDF HTML
  Cite this Reference:
N.R. Dhanutha, M.R. Reddy and S.S. Lakshman Rao, 2012. Evidence of Avian Leukosis Virus Subgroup E and Endogenous Avian Virus in Marek’s Disease Vaccines Derived from Chicken Embryo Fibroblasts.  International Journal of Animal and Veterinary Advances, 4(6): 363-369.
    Advertise with us
ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
Submit Manuscript
   Current Information
   Sales & Services
   Contact Information
  Executive Managing Editor
  Email: admin@maxwellsci.com
  Publishing Editor
  Email: support@maxwellsci.com
  Account Manager
  Email: faisalm@maxwellsci.com
  Journal Editor
  Email: admin@maxwellsci.com
  Press Department
  Email: press@maxwellsci.com
Home  |  Contact us  |  About us  |  Privacy Policy
Copyright © 2009. MAXWELL Science Publication, a division of MAXWELLl Scientific Organization. All rights reserved