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2012 (Vol. 4, Issue: 3)
Article Information:

Determination Melatonin in Serum of Kurdish Horses by HPLC in Kermanshah Region at Breeding Season

Afsaneh Arabi and Hossien Najafzadeh
Corresponding Author:  Hossien Najafzadeh 

Key words:  HPLC, kermanshah region, kurdish horse, melatonin, , ,
Vol. 4 , (3): 170-172
Submitted Accepted Published
December 23, 2011 January 16, 2012 June 15, 2012

The objective of this study was to evaluate changes in serum concentration of melatonin of Kurdish horses Kermanshah region at breeding season (February to June of 2010). Blood samples from jugular vein of 40 Kurdish horses were collected. Serum was harvested from these blood samples by centrifugation. After preparation and derivation of serums for HPLC assay, samples were injected to HPLC column and melatonin was measured by area under curve based on standard curve of melatonin. The mean (S.E) of serum melatonin was determined 63.239.51 pg/mL. The concentration of serum melatonin of mares was nearly 2 times than stallions and significantly differed (p = 0.01). Thus serum melatonin related to breed of horses and may affect reproductive activity in different breed and geographical region.
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  Cite this Reference:
Afsaneh Arabi and Hossien Najafzadeh, 2012. Determination Melatonin in Serum of Kurdish Horses by HPLC in Kermanshah Region at Breeding Season.  International Journal of Animal and Veterinary Advances, 4(3): 170-172.
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ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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