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Abstract
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Article Information:
Determination Melatonin in Serum of Kurdish Horses by HPLC in Kermanshah Region at Breeding Season
Afsaneh Arabi and Hossien Najafzadeh
Corresponding Author: Hossien Najafzadeh
Submitted: December 23, 2011
Accepted: January 16, 2012
Published: June 15, 2012 |
Abstract:
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The objective of this study was to evaluate changes in serum concentration of melatonin of Kurdish
horses Kermanshah region at breeding season (February to June of 2010). Blood samples from jugular vein of
40 Kurdish horses were collected. Serum was harvested from these blood samples by centrifugation. After
preparation and derivation of serums for HPLC assay, samples were injected to HPLC column and melatonin
was measured by area under curve based on standard curve of melatonin. The mean (±S.E) of serum melatonin
was determined 63.23±9.51 pg/mL. The concentration of serum melatonin of mares was nearly 2 times than
stallions and significantly differed (p = 0.01). Thus serum melatonin related to breed of horses and may affect
reproductive activity in different breed and geographical region.
Key words: HPLC, kermanshah region, kurdish horse, melatonin, , ,
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Abstract
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Cite this Reference:
Afsaneh Arabi and Hossien Najafzadeh, . Determination Melatonin in Serum of Kurdish Horses by HPLC in Kermanshah Region at Breeding Season. International Journal of Animal and Veterinary Advances, (3): 170-172.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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