The aims of this work were to study the relationship between temperature and hatching rate and to evaluate genetic diversity of giant frog (Limnonectes blythii) as parental stock in Mae Hong Son Inland Fisheries Station, northern Thailand. The results showed that the optimal temperature for development of giant frog hatching ranged from 19 to 27ºC, based on total hatch rate, viability of newly hatched larvae post-hatch, and total mortality rate of eggs. Moreover, from November to February, the greatest percentage of hatching was in January, with 40%. The outcome of determining the genetic diversity of parental generation by using seven microsatellite loci was moderately high levels.