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Abstract
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Article Information:
Field Isolation of Mycoplasma capripneumoniae in Central Zone of Tanzania
E.Y. Noah, L.J.M. Kusiluka, P. Wambura and S.I. Kimera
Corresponding Author: E.Y. Noah
Submitted: 2011 October, 12
Accepted: 2011 November, 25
Published: 2011 December, 25 |
Abstract:
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An investigation was done on outbreak of acute respiratory disease in goats in Central zone of
Tanzania, the investigation involved 3 districts. (Mpwapwa, Dodoma and Kilosa districts). The study involved
goats from the field, livestock markets and abattoirs. Samples from clinical and pathological examination of
goats with respiratory distress were subjected to microbiological and molecular analysis. A total of 96 lungs
samples, 96 lymph node and 59 pleural fluids were collected for microbiological and molecular evaluation.
Media used were Hayflick’s medium (H25P) and commercial freeze-dried diagnostic medium for mycoplasma
isolation, blood and MacConkey agar for bacteria other than mycoplasma. Mycoplasma isolates were confirmed
by Polymerase Chain Reaction (PCR) and Restriction Enzyme Analysis (REA). Mycoplasma capripneumoniae
was isolated from 47 samples and Pasteurella multocida from 13 samples. Eighty one samples subjected to
molecular analysis were positive for M. capripneumoniae. M. capripneumoniae was isolated from samples
collected from all the study districts. The results of this study indicate that outbreaks of respiratory disease in
the study areas were predominantly due to contagious caprine pleuropneumonia (CCPP). This provides
additional information on the distribution of the disease in the country. The results of PCR/REA demonstrate
the usefulness of this diagnostic technique in the investigation of field outbreaks of CCPP even when isolation
of the causative agent is not possible. The isolation and identification of M. capripneumoniae in central zone
confirm the presence of contagious caprine pleuropneumonia in the zone.
Key words: Goats, Mycoplasma capripneumonia, polymarase chain reaction, restriction enzymes, , ,
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Cite this Reference:
E.Y. Noah, L.J.M. Kusiluka, P. Wambura and S.I. Kimera, . Field Isolation of Mycoplasma capripneumoniae in Central Zone of Tanzania. International Journal of Animal and Veterinary Advances, (6): 434-442.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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