The objective of this study was to determine the influence of degradation characteristics of six feed
supplements on ruminal volatile fatty acid and amino acid production and profile using three ruminally
cannulated male sheep. The sheep were fed a concentrate to forage ratio of 35:65. The supplements came from
Soybean Meal (SBM), Corn Meal (CM), Meat and Bone Meal (MBM), Feather Meal (FtM), Blood Meal (BM),
and distillers Dried Gain with Soluble (DDGs). Sheep were fed at 6 h interval for 42 days. Ruminal parameters
such as pH, NH4+, temperature, NO3-, and Volatile Fatty Acids (VFA) were measured over a 12 h period after
feeding. Concentrations of pH, NH4+, and NO3- showed significant difference (p<0.001) across all dietary
treatments. Sheep fed BM had higher pH (6.64 vs. 5.81), NH4+ (262.31 vs. 1.95 ppm), and NO3- (559.71 vs.
77.40 ppm) values than did those fed CM whereas FtM showed intermediate values. The C2/ (C3+C4) ratio of
the major VFA showed a significant difference (p<0.001). Ruminal profile (%) of the sum of amino acids
measured was similar among feeds, except Asp, isoleucine, lysine, and proline, respectively. Specific first
limiting AA was methionine for SBM while lysine was co-limiting for FtM and DDGs. In general, ruminal
parameters were clearly influenced by dietary treatments, with a lower ruminal pH and a higher NH4+ in BM
than CM. Overall, the concentration of both total EAA and NEAA were similar among feeds, but numerically
SBM had more EAA concentrations followed by CM, MBM, DDGs, BM, and FtM.