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Abstract
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Article Information:
Characterization of the dUTpase- Integrase Region of the Pol Gene of Small Ruminant Lentiviruses from Greece
Y.M. Eltahir
Corresponding Author: Yassir Mohammed ELtahir
Submitted: 2011 April, 01
Accepted: 2011 May, 10
Published: 2011 June, 10 |
Abstract:
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Despite the wide spread of Small Ruminant’s Lentiviruses (SRLV), Maedi Visna Virus (MVV) and
caprine arthritis-encephalitis (CAEV) in Greece, sequence information on the pol gene of Greek SRLV
(GrSRLV) is not reported before. The present study was aimed to establish a sequence database in a conserved
region of the viral genome which can then serve to perform phylogenetic analysis. Three hundred and seventy
six nucleotides (dUTPase- integrase) region of the pol gene of GrSRLV from different mixed flocks were
obtained by semi-nested PCR using degenerate primers. GrSRLV showed 79 and 81% homology in their
nucleotides and deduced amino acids sequences respectively. Molecular epidemiology based on the pol
sequences indicated that GrSRLV contains both type A and B viruses and the occurrence of five genetically
different clusters of SRLV circulating in Greece. The results suggest that GrA-SRLV have a different origin
from that of British, Icelandic and South African SRLV reference strains. It also gives additional evidence of
interspecies transmission of these viruses between sheep and goats.
Key words: Caprine arthritis encephalitis, maedi visna, phylogeny, pol, small ruminat lentiviruses, ,
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Abstract
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Cite this Reference:
Y.M. Eltahir, . Characterization of the dUTpase- Integrase Region of the Pol Gene of Small Ruminant Lentiviruses from Greece. International Journal of Animal and Veterinary Advances, (3): 170-176.
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ISSN (Online): 2041-2908
ISSN (Print): 2041-2894 |
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