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    Abstract
2011 (Vol. 3, Issue: 3)
Article Information:

Characterization of the dUTpase- Integrase Region of the Pol Gene of Small Ruminant Lentiviruses from Greece

Y.M. Eltahir
Corresponding Author:  Yassir Mohammed ELtahir 

Key words:  Caprine arthritis encephalitis, maedi visna, phylogeny, pol, small ruminat lentiviruses, ,
Vol. 3 , (3): 170-176
Submitted Accepted Published
2011 April, 01 2011 May, 10 2011 June, 10
Abstract:

Despite the wide spread of Small Ruminantís Lentiviruses (SRLV), Maedi Visna Virus (MVV) and caprine arthritis-encephalitis (CAEV) in Greece, sequence information on the pol gene of Greek SRLV (GrSRLV) is not reported before. The present study was aimed to establish a sequence database in a conserved region of the viral genome which can then serve to perform phylogenetic analysis. Three hundred and seventy six nucleotides (dUTPase- integrase) region of the pol gene of GrSRLV from different mixed flocks were obtained by semi-nested PCR using degenerate primers. GrSRLV showed 79 and 81% homology in their nucleotides and deduced amino acids sequences respectively. Molecular epidemiology based on the pol sequences indicated that GrSRLV contains both type A and B viruses and the occurrence of five genetically different clusters of SRLV circulating in Greece. The results suggest that GrA-SRLV have a different origin from that of British, Icelandic and South African SRLV reference strains. It also gives additional evidence of interspecies transmission of these viruses between sheep and goats.
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  Cite this Reference:
Y.M. Eltahir, 2011. Characterization of the dUTpase- Integrase Region of the Pol Gene of Small Ruminant Lentiviruses from Greece.  International Journal of Animal and Veterinary Advances, 3(3): 170-176.
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ISSN (Online):  2041-2908
ISSN (Print):   2041-2894
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