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2011 (Vol. 3, Issue: 1)
Article Information:

Comparative Studies of the Spectrophotometric and Microbiological Assay Methods Used for the Determination of Ampicillin Levels in Ampicillin/Cloxacillin Combinations

C.E. Udobi, P.M.E. Ubulom and E.I. Akpabio
Corresponding Author:  Chinweizu Ejikeme Udobi 

Key words:  Ampicillin, cloxacillin, Infra red, spectrophotometric assay, spectroscopy, ,
Vol. 3 , (1): 31-34
Submitted Accepted Published
2010 October, 16 2010 December, 02 2011 January, 15
Abstract:

The spectrophotometric and microbiological assay methods used for the determination of ampicillin levels in ampicillin/cloxacillin combinations were compared. A total of 24 samples of am picillin/cloxacillin suspensions from 8 batches and 3 companies (B2, D and E) were assayed for their ampicillin content levels using the Spectrophotometric assay method. Infra-red (IR) Spectroscopy confirm ed the presence of ampicillin in all the samples and the results obtained showed that the samples had a wide range of amounts of ampicillin which compared favourably w ith the microbiological assay results of the sam e products. A ll the products assayed had ampicillin content within acceptable ranges of the BP and USP. Spectrophotometric assay is recommended for the quick determination of the levels of ampicillin in their combinations with cloxacillin.
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  Cite this Reference:
C.E. Udobi, P.M.E. Ubulom and E.I. Akpabio, 2011. Comparative Studies of the Spectrophotometric and Microbiological Assay Methods Used for the Determination of Ampicillin Levels in Ampicillin/Cloxacillin Combinations.  Current Research Journal of Biological Sciences, 3(1): 31-34.
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