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2011 (Vol. 3, Issue: 1)
Article Information:

Isolation and Purification of L-amino Acid Oxidase from Indian Cobra Naja naja

P. Dineshkumar and B. Muthuvelan
Corresponding Author:  B. Muthuvelan 

Key words:  Indian cobra, L-amino acid oxidase, Naja naja, purification, , ,
Vol. 3 , (1): 6-11
Submitted Accepted Published
2010 June, 26 2010 July, 31 2011 January, 15
Abstract:

The enzyme L-Amino Acid Oxidase (LAAO) is widely distributed in snake venoms, contributes a reasonable toxicity to the venoms. However LAAO from Indian cobra (Naja naja) snake venom has not yet isolated previously. In the present study LAAO from Indian cobra (Naja naja) snake venom was purified by sequential steps of cation exchange chromatography (CM-Sephadex C-25; CM-52 cellulose), followed by sephadex G-100 gel filtration chromatography. The purified enzyme was named as Indian Cobra Naja naja LAmino Acid Oxidase (ICN-LAAO). This ICN-LAAO is a monomer and its molecular mass has been found to be 61.91 kDa determined by MALDI-TOF and 12% SDS-PAGE. The enzyme has an isoelcetric point of 8.12 and a pH optimum of 8.5. It shows remarkable thermal stability and partial inactivation by freezing. The enzyme may contribute in the development of severe hematological disorder due to cobra venom envenomation.
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  Cite this Reference:
P. Dineshkumar and B. Muthuvelan, 2011. Isolation and Purification of L-amino Acid Oxidase from Indian Cobra Naja naja.  Current Research Journal of Biological Sciences, 3(1): 6-11.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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