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Abstract
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Article Information:
Studies on Isolation, Classification and Phylogenetic Characterization of Novel Antifungal Streptomyces sp. VITSTK7 in India
M. Thenmozhi and K. Kannabiran
Corresponding Author: K. Kannabiran
Submitted: 2010 June, 28
Accepted: 2010 July, 30
Published: 2010 September, 15 |
Abstract:
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The aim of the study was to screen the antifungal activity of the crude extract prepared from the
strain Streptomyces spp. VITSTK7 against Aspergillus sp. and to characterize the isolate. A total of 8 strains
were isolated from the marine sediments collected at the Puducherry coast, India. All the eight strains were
primarily screened for antifungal activity against three species of Aspergillus namely A. fumigatus, A. niger and
A. flavus. Our search resulted in the isolation of a potential strain VITSTK7. The production media was
optimized for maximum yield of secondary metabolites. The metabolites were extracted using ethyl acetate,
lyophilized and screened for antifungal activity against the three Aspergillus species by well diffusion method.
A maximum zone of inhibition (21 mm) was observed for A. fumigatus in comparison with the standard
antifungal antibiotic Nystatin (20 mm). This potential strain was further identified based on Hideo Nonomura
classification. A phylogenetic tree was constructed by maximum parsimony method to identify up to the species
level. Molecular taxonomy and phylogeny revealed that the strain belonged to the genus Streptomyces and was
designated as Streptomyces spp.VITSTK7. Blast search of the 16s rRNA sequence of the strain with the
sequences available in the NCBI data bank showed a maximum similarity of 86% with Streptomyces
longisporoflavus (DQ 442520) with the bootstrap value of 100. The 16s rRNA sequence of the strain
Streptomyces spp.VITSTK7 was submitted to the GenBank under the accession number GQ 499369. The
secondary structure of 16s rRNA and the restriction sites were also predicted using Genebee and NeBCutter
online softwares, respectively.
Key words: Actinomycetes, antifungal activity, Aspergillus fumigatus, Streptomyces spp. VITSTK7, , ,
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Cite this Reference:
M. Thenmozhi and K. Kannabiran, . Studies on Isolation, Classification and Phylogenetic Characterization of Novel Antifungal Streptomyces sp. VITSTK7 in India. Current Research Journal of Biological Sciences, (5): Page No: 306-312.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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