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    Abstract
2013 (Vol. 5, Issue: 6)
Article Information:

Genomic Island Location in Acinetobacter baumannii Strains by tRIP-PCR Technique

Suhadsaad, Alice K. Melconian, Ali H. Ad'hiah and Kumar Rajakumar
Corresponding Author:  Suhadsaad 

Key words:  Acinetobacter baumannii, genomic islands, tRIP-PCR, , , ,
Vol. 5 , (6): 296-301
Submitted Accepted Published
August 01, 2013 August 26, 2013 November 20, 2013
Abstract:

This study was performed to detect the presence of genomic islands which usually insert in the tRNA genes and other non-coding RNA genes, in this study eight strains of Acinetobacter baumannii (AYE, A457, A14, A424 A473, A92, ACICU, A25) were tested by used of tRIP-(tRNA site Interrogation for Pathogeni city islands, prophases and other GIs)-PCR method. The results of PCR and agarose gel electrophoresis for eight strains of two loci #7, #24 were: the results of #7 loci screening showed that all strains were positive except A. baumannii 457 strain was negative. While the results of #24 loci showed presence of foreign DNA in A. baumannii AYE, A457, A14, A424, A473, A92 except the results of (ACICU, A25) was positive.
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  Cite this Reference:
Suhadsaad, Alice K. Melconian, Ali H. Ad'hiah and Kumar Rajakumar, 2013. Genomic Island Location in Acinetobacter baumannii Strains by tRIP-PCR Technique.  Current Research Journal of Biological Sciences, 5(6): 296-301.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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