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2013 (Vol. 5, Issue: 4)
Article Information:

Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using PCR Technique

Hadeel Waleed Abdulmalk
Corresponding Author:  Hadeel Waleed Abdulmalk 

Key words:  Pleurotus ostereatus, Pleurotus sapidus, Higher Fungi, PCR; (ITS) region primers, RAPD-PCR, Edible mushrooms,
Vol. 5 , (4): 157-160
Submitted Accepted Published
November 19, 2012 January 21, 2013 July 20, 2013
Abstract:

Pleurotus ostreatus and Pleurotus sapidus are difficult to differentiate using standard morphologically based characteristics. This study, using two taxon-selective primers for the Internal Transcribed Spacer (ITS) region in the nuclear ribosomal repeat unit. These primers, ITS1-F & ITS4, were intended to be specific for the higher fungi, respectively. RAPD-PCR technique was used to reveal DNA polymorphism in DNA of the two fungi Pleurotus ostreatus and Pleurotus sapidus in order to search for the sources of differences that could be used as a DNA marker represent the differentiation between this two species. In RAPD-PCR, eight different decamer primers chosen randomly were employed to detect the genetic polymorphisms among studied strains.
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  Cite this Reference:
Hadeel Waleed Abdulmalk, 2013. Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using PCR Technique.  Current Research Journal of Biological Sciences, 5(4): 157-160.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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