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Abstract
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Article Information:
Manipulation of Ovaries/Ovules and Clearance and Isolation of Embryo Sacs of Impatiens glandulifera and Nicotiana tabacum
Noreldaim Hussein
Corresponding Author: Noreldaim Hussein
Submitted: June 19, 2012
Accepted: July 09, 2012
Published: May 20, 2013 |
Abstract:
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The objective of this research was to develop techniques to clear and/or isolate embryo sacs of Impatiens glandulifera and Nicotiana tabacum, to facilitate microscopic examination for subsequent experimentation on in vitro fertilization of flowering plants. Two techniques were applied: The clearing and enzymic maceration. Both of the 2 adopted techniques were effective in revealing embryo sacs of the studied plant species. A fixative prepared from propionic acid, formaldehyde, ethanol (50%) in the ratio 5:5:90 was used. The clearing solution was prepared from lactic acid (85%), chloral hydrate, phenol, clove oil and xylene in the ratio 2:2:2:2:1 by weight. Both the clearing and the enzymic maceration techniques proved effective in revealing embryo sacs of I. glandulifera and N. tabacum
Key words: Embryo sac, enzymic maceration, fixation techniques, ovules, , ,
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Cite this Reference:
Noreldaim Hussein, . Manipulation of Ovaries/Ovules and Clearance and Isolation of Embryo Sacs of Impatiens glandulifera and Nicotiana tabacum. Current Research Journal of Biological Sciences, (3): 91-95.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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