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2013 (Vol. 5, Issue: 3)
Article Information:

Manipulation of Ovaries/Ovules and Clearance and Isolation of Embryo Sacs of Impatiens glandulifera and Nicotiana tabacum

Noreldaim Hussein
Corresponding Author:  Noreldaim Hussein 

Key words:  Embryo sac, enzymic maceration, fixation techniques, ovules, , ,
Vol. 5 , (3): 91-95
Submitted Accepted Published
June 19, 2012 July 09, 2012 May 20, 2013
Abstract:

The objective of this research was to develop techniques to clear and/or isolate embryo sacs of Impatiens glandulifera and Nicotiana tabacum, to facilitate microscopic examination for subsequent experimentation on in vitro fertilization of flowering plants. Two techniques were applied: The clearing and enzymic maceration. Both of the 2 adopted techniques were effective in revealing embryo sacs of the studied plant species. A fixative prepared from propionic acid, formaldehyde, ethanol (50%) in the ratio 5:5:90 was used. The clearing solution was prepared from lactic acid (85%), chloral hydrate, phenol, clove oil and xylene in the ratio 2:2:2:2:1 by weight. Both the clearing and the enzymic maceration techniques proved effective in revealing embryo sacs of I. glandulifera and N. tabacum
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  Cite this Reference:
Noreldaim Hussein, 2013. Manipulation of Ovaries/Ovules and Clearance and Isolation of Embryo Sacs of Impatiens glandulifera and Nicotiana tabacum.  Current Research Journal of Biological Sciences, 5(3): 91-95.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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