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Abstract
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Article Information:
Immunomodulating Effect of Chitosan Extracted from Aspergillus niger
Reema Mohammed Abed Aloubaidy
Corresponding Author: Reema Mohammed Abed Aloubaidy
Submitted: October 17, 2012
Accepted: November 23, 2012
Published: January 20, 2013 |
Abstract:
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This study was conducted to extract chitosan from Aspergillus niger mycelia using hot alkaline followed by acid treatment and after 12 days of fermentation in sabouro dextrose broth containing 8% glucose, 1.246 g/L of chitosan was obtained. Five male albino Swiss mice were injected intraperitoneally with constant dose of chitosan (1 mg/mL) twice during two weeks and then blood picture, microscopic examination and double diffusion test were carried out. Chitosan was reported to increase the number of cells which were 530, 250 and 1237%, for Lymphocyte, Granulocytes and monocyte respectively when compared with control group. Also mice organs were crossly examined and they showed hypertrophy of liver and spleen, while microscopic examination reveled that spleen diffuse hyperplasia and infiltrate of mononuclear cells and megakaryocte. Moreover no antibody was detected in the serum of treated animals against chitosan.
Key words: Aspergillus niger, chitosan, immunization, immunomodulator, leukocyte, polysaccharide,
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Abstract
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Cite this Reference:
Reema Mohammed Abed Aloubaidy, . Immunomodulating Effect of Chitosan Extracted from Aspergillus niger. Current Research Journal of Biological Sciences, (2): 58-61.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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