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    Abstract
2012 (Vol. 4, Issue: 4)
Article Information:

Malaria-Induced Apoptosis in the Cells of Interlukine-6Rα knockout Mice

Saad Hussin Alkahtani
Corresponding Author:  Saad Alkahtani 

Key words:  Apoptosis, IL-Rα and spleen, liver, malaria, mice, ,
Vol. 4 , (4): 500-506
Submitted Accepted Published
April 12, 2012 April 25, 2012 July 10, 2012
Abstract:

The purpose of this study was to illustrate the function of IL-6Rα during malaria infection by investigating the responses of Plasmodium chabaudi malaria in hepatocytes and spleenocytes of IL-6Rα+/+ and IL-6Rα-/- female C57BL/6 mice via evaluation of mRNA expression of apoptotic genes (Bax, Bcl-2 and Caspase-3). Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then scarified at days (0, 1, 4 and 8, respectively). RT-PCR was used to quantify apoptotic genes level. In the IL- 6Rα+/+ the levels of liver Bax were significantly increased at days 1 and 8 while they were significantly decreased only at day 1 in the IL-6Rα--/- compared with day 0. In contrast, the IL-6Rα+/+ levels of spleen Bax were significantly increased only at day 8. In the IL-6Rα+/+ the levels of liver Bcl-2 were significantly increased at all days after infection while significantly decreased only at day 1 in the spleen IL-6Rα-/- compared with day 0. In the IL-6Rα+/+ the levels of liver caspase-3 were significantly increased at days 1 and 8 while significantly decreased in the IL-6Rα-/- at day 8 compared with day 0. However, in the IL-6Rα+/+ the levels of spleen caspase-3 were significantly increased at day 8 only while significantly decreased at day 8 in the IL- 6Rα-/-compared with day 0. In conclusion, this data has shown that the absence of IL-6Rα plays an important role in decreasing the severity of apoptosis during malaria disease. Thus, this study suggests that after further investigation the role of IL-6Rα could be the therapeutic key for determining the outcome of malaria in liver cells.
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  Cite this Reference:
Saad Hussin Alkahtani, 2012. Malaria-Induced Apoptosis in the Cells of Interlukine-6Rα knockout Mice.  Current Research Journal of Biological Sciences, 4(4): 500-506.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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