In order to investigate the properties of pepper fruit at different substrates as a factorial experiment in completely randomized design with four replications. The experiment was carried out in the Islamic Azad University, Jiroft branch, Iran, in 2011. Treatments included three green pepper cultivars (Sereno, LiricaRZ and Figaro) and three substrates of vermiculite + sand, peat + perlite and rock wool. Result showed that the most yield and average of fruit weight was 2003.91 and 129.14 g, respectively and number of fruit with average of 15.5, fruit length with 10.85 cm were obtained from substrate of peat + perlite. LiricaRZ had the most yield and average of fruit weight 1498.97 and 122.41 g, respectively than two other green peppers. There was significant interaction effect between green pepper and substrate in fruit's weight at 5% and fruit's length at 1%. It was known that the best substrate was peat + perlite and the best green pepper cultivar was LiricaRZ in hydroponic cultivation.