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    Abstract
2012 (Vol. 4, Issue: 4)
Article Information:

Stress Response in Infective Larvae (L3) of the Parasitic Nematode Haemonchus contortus is Accompanied by Enhanced Expression of Heat Shock Proteins (HSP 70)

D.N. Siamba, P.N. Gatongi, C.S. Mulambalah, M.M. Ngeiywa and L.W. Wamae
Corresponding Author:  Siamba 

Key words:  Haemonchus, heat shock proteins, moisture, stress, temperature, ,
Vol. 4 , (4): 345-349
Submitted Accepted Published
April 04, 2011 May 13, 2011 July 10, 2012
Abstract:

The study was undertaken to investigate the effects of temperature and moisture stresses on protein profile and characteristics in the free living third larval stages (L3) of Haemonchus contortus. Before and after temperature and moisture stress treatment, the L3 were tested for the induction and expression of Heat-Shock Proteins (HSPs). Proteins were analyzed by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and immunoblot analysis with alkaline-phosphatase conjugated anti HSP 70 antibody. The stress resulted in an altered protein pattern. It was also revealed that stress treatment did not alter the signals obtained with the anti HSP 70 antibody, but the amount of HSP70 as estimated by densitometry differed between pre and post stress samples. This response in the free living third stage larvae (L3) of H. contortus may be aimed at protecting the parasite against molecular damage and ensuring survival during stressful periods.
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  Cite this Reference:
D.N. Siamba, P.N. Gatongi, C.S. Mulambalah, M.M. Ngeiywa and L.W. Wamae, 2012. Stress Response in Infective Larvae (L3) of the Parasitic Nematode Haemonchus contortus is Accompanied by Enhanced Expression of Heat Shock Proteins (HSP 70).  Current Research Journal of Biological Sciences, 4(4): 345-349.
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ISSN (Online):  2041-0778
ISSN (Print):   2041-076X
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