|
Abstract
|
Article Information:
Evaluation of Genetic Diversity in Three Species of Retama Genus: R. monosperma (L) Boiss, R. raetam (Forssk) Webb and R. sphaerocarpa (L) Boiss. (Fabaceae) Based on SDS-PAGE
Belmokhtar Zoubir and Kaid- Harche Meriem
Corresponding Author: Belmokhtar Zoubir
Submitted: 2011 November, 25
Accepted: 2011 December, 18
Published: 2012 March, 10 |
Abstract:
|
Genetic interpretation of protein polymorphism in Retama genus has not been well established until
now. The aim of this study was to investigate interspecific and intraspecific polymorphism in three Species of
Retama Genus: R. monosperma (L) Boiss, R. raetam (Forssk) Webb and R. sphaerocarpa (L) Boiss, by sodium
dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE). In this study twelve populations collected
from different regions of Algeria have been studied for the analysis of seed storage protein profiles to examine
their relationship. Based on electrophoresis bans spectra, Jaccard’s Similarity Index was calculed and cluster
analysis was performed using unweighted pair group method with arithmetical means (UPGMA), to check the
polymorphism among populations. The results from the present study indicate that the low polymorphism
between R. monosperma and R. raetam. The cluster analysis indicated that the twelve populations constituted
two clusters. Cluster A comprised from all population of R. monosperma and R. raetam, and Cluster B
comprised of 2 populations from R. sphaerocarpa. The SDS banding proteins pattern showed that four bands
are monomorphic in all populations of three species with molecular weights of 31.761, 63.767, 92.363 and 82.832 KDa.
Key words: Electrophoresis, polymorphism, SDS-PAGE, R. monosperma, R. raetam, R. sphaerocarpa,
|
|
Abstract
|
PDF
|
HTML |
|
Cite this Reference:
Belmokhtar Zoubir and Kaid- Harche Meriem, . Evaluation of Genetic Diversity in Three Species of Retama Genus: R. monosperma (L) Boiss, R. raetam (Forssk) Webb and R. sphaerocarpa (L) Boiss. (Fabaceae) Based on SDS-PAGE. Current Research Journal of Biological Sciences, (2): 202-205.
|
|
|
|
 |
ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
 |
| Information |
|
|
|
|
| Sales & Services |
|
|
|
|
