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Abstract
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Article Information:
Survey of Layer Flocks Contamination to Mycoplasma gallisepticum in East Azerbaijan Province by Rapid Slide Agglutination (R.S.A) Method
Adel Feizi and Mehrdad Nazeri
Corresponding Author: Adel Feizi
Submitted: 2011 October, 23
Accepted: 2011 November, 25
Published: 2012 January, 20 |
Abstract:
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The objective of this study was to evaluation of R.S.A. method efficiency in distinguishing
M. gallisepticum and contamination rate of MG in Iran. In this study totally 300 serum samples from 20 farms
collected and sent to laboratory. In lab, samples centrifuged at 2500 rpm for 5 min. Separated serums kept in
Bain Marie at 56ºC. Then 50 μ of serum are mixed with 50 : RSA-MG antigen on the slide and were assayed
under light. Of 300 serum samples, 52 samples (17.3%) were positive, 10 samples (3.3%) were suspicious and
238 samples (79.3%) were negative reported. Finally, primarily can be conclude that R.S.A method is specific
method for detection of M. gallisepticum and has minimum Err with maximum sensitivity. Secondarily can be
conclude that contamination rate of M. gallisepticum in Iran is higher than standard levels and must be take
measures in this field.
Key words: Layer flocks, Mycoplasma gallisepticum, R.S.A, Tabriz, , ,
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Cite this Reference:
Adel Feizi and Mehrdad Nazeri, . Survey of Layer Flocks Contamination to Mycoplasma gallisepticum in East Azerbaijan Province by Rapid Slide Agglutination (R.S.A) Method. Current Research Journal of Biological Sciences, (1): 48-51.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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