The antibacterial, antioxidant activities and the phytochemical analysis of Sesbania rostrata used in traditional medicine in Burkina Faso were investigated. Aqueous, methanolic and hydro-acetone extracts from leaves, stems, granules, pods and roots organs have demonstrated a good polyphenolic, tannin and flavonoids with variable anti-DPPH, Iron III reduction and antibacterial capacities. Particularly methanol extract form leaves possessed 46.33 mgEGA/100 mg and 25.98 mgETA/100 mg in polyphenolic and tannin content respectively. Beside TLC analysis of this extract demonstrated the presence of quercetin, kaempferol, rutin, caffeic and gallic acids. It was presented a good possibility to inhibit bacteria growth, radical DPPH and to reduce Iron III. These biological activities could support the traditional uses of this plant.