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2011 (Vol. 3, Issue: 5)
Article Information:

The Effects of Different Concentrations and Combinations of Growth Regulators on the Callus Formation of Potato (Solanum tubrosum) Explants

Shahab-ud-din, I.N. Sultan, M.A. Kakar, A. Yousafzai, F.A. Sattar, F. Ahmmad, S.M. Ibrahim, M. Hassanullah and B. Arif
Corresponding Author:  Shahab-ud-din 

Key words:  Callus, explants, plant growth regulators, potato, , ,
Vol. 3 , (5): 499-503
Submitted Accepted Published
2011 June, 27 2011 August, 08 2011 September, 10
Abstract:

The main objective of the present study was to investigate the effects of different concentrations of plant growth regulators and their combinations on callus induction of potato (Solanum tuberosum L.). The explants of potato tuber were cultured on Modified Murashige and Skoog medium which was supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene Acetic Acid (NAA), Benzyl Adenine (BA), 2,4-D in combinations with BA and NAA in combination with BA for callus induction. The Concentration of sucrose was 3% W/V level and the pH of the media was adjusted to 5.7 before the addition of agar 8% W/V. The explants were first dissected out aseptically and then inoculated to the media (with various levels of hormones), then incubated at 27±2șC in the culture room. Among the treatments 2, 4-D at different concentrations produced different degree of callus but comparatively a massive amount of callus was formed on MS medium supplemented with 2, 4-D alone at 3.0 mg/L. Also NAA and BA with different concentrations produced considerable degrees of callus but the degree of callus was best at higher concentrations of NAA and BA. 2, 4-D in combination with BA at 2.0 mg/L both produced considerable amount of callus. In case of NAA in combination with BA the degree of callus formation was best at concentration 1.0 mg/L each. So according to the above findings it was concluded that 2, 4-D is the best option for induction of callus among the other hormones used in the study.
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  Cite this Reference:
Shahab-ud-din, I.N. Sultan, M.A. Kakar, A. Yousafzai, F.A. Sattar, F. Ahmmad, S.M. Ibrahim, M. Hassanullah and B. Arif, 2011. The Effects of Different Concentrations and Combinations of Growth Regulators on the Callus Formation of Potato (Solanum tubrosum) Explants.  Current Research Journal of Biological Sciences, 3(5): 499-503.
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