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Abstract
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Article Information:
In Search of Target Gene(s) to Quantify Pea Pathogenic Nectria haematococca in Agricultural Soils
Ebimieowei Etebu and A. Mark Osborn
Corresponding Author: Ebimieowei Etebu
Submitted: 2011 February, 04
Accepted: 2011 March, 08
Published: 2011 May, 05 |
Abstract:
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Footrot disease due to N. haematococca (anamorph Fusarium solani f. sp. pisi) is a globally,
economically important disease of peas. The disease has been linked to the presence of six pea pathogenicity
(PEP) genes (PDA1, PEP1, PEP2, PEP3, PEP4 and PEP5) inherent in pathogenic forms of the causal fungus
N. haematococca MPIV. The disease is prevented only through avoidance of fields with high disease potential.
Identifying agricultural fields with a high disease potential prior to pea cultivation has been paramount in the
implementation of preventive measures. Although molecular techniques have been successfully used to quantify
pathogenic strains of N. haematococca in agricultural soils, targeting all six pathogenicity genes in these assays
would not be cost effective. This study therefore attempts to review the functions and roles of the different
genes linked with pea pathogenicity with the aim of identifying gene(s) that would serve as a logical target in
a quantitative molecular assay. Findings suggest that, whilst the PDA gene may be targeted in a preliminary
diagnostic measure, a conclusive assay, targeting the PEP3 gene may be required to affirm pea footrot disease
potential of agricultural fields. Agricultural fields with the PEP3 gene copy numbers of up to 100 per g soil
prior to cultivation may be deemed unsafe for peas.
Key words: Footrot disease, Fusarium solani f. sp. pisi, Nectria haematococca, pathogenicity genes, pea, Pisum sativum,
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Cite this Reference:
Ebimieowei Etebu and A. Mark Osborn, . In Search of Target Gene(s) to Quantify Pea Pathogenic Nectria haematococca in Agricultural Soils. Current Research Journal of Biological Sciences, (3): 195-208.
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ISSN (Online): 2041-0778
ISSN (Print): 2041-076X |
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