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Abstract
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Article Information:
Essential Oil of Syzygium samarangense; A Potent Antimicrobial and Inhibitor of Partially Purified and Characterized Extracellular Protease of Escherichia coli 25922
S. Adeola Adesegun, O. Folorunso Samuel, B. Ojekale Anthony, B. Ogungbe Folasade and S. Kayode Mary
Corresponding Author: S. Adeola Adesegun
Submitted: February 16, 2013
Accepted: March 11, 2013
Published: December 25, 2013 |
Abstract:
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Volatile oils being secondary metabolites are phytoactive ingredients found in medicinal plants and may be active against various infectious microorganisms. The present study was carried out to evaluate the antimicrobial effect of the volatile oil from the leaf of Syzygium samarangense on Escherichia coli and its inhibition on the extracellular protease of this organism. The volatile oil inhibited the growth of Escherichia coli with IC50 of 0.42% (v/v). The extracellular protease of this organism exhibited highest activities at pH 7.0 and 43°C. This enzyme was moderately activated by the chloride salts of Zn2+, K+ and Cu2+. The set of chloride salts of Ba2+, Pb 2+, Hg2+, and Mg2+, Mn2+, Co2+, Ca2+, Fe2+ were, respectively strong and mild inhibitors against the activity of this enzyme. The line weaver burke kinetic plot indicated a competitive mode of inhibition by the volatile oil on the enzyme with Vmax of 8.33×103 &mumol/min and the Km in the absence and presence of the volatile oil (inhibitor) were 0.23 mg/mL and 1.25 mg/mL, respectively. The highest percentage yield during purification was 66.3 and the highest purification fold was11.9 as compared to the crude enzyme. Sephadex G-100 gel filtration produced one peak each for total protein and enzyme activity. Therefore, the volatile oil from the leaf of Syzygium samarangense may possess antimicrobial activity and its inhibitory effect on the extracellular protease of Escherichia coli may be one of its modes of action on the pathogenic organisms.
Key words: Antimicrobial, Escherichia coli, extracellular protease, inhibitor, Syzygium samarangense, volatile oil,
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Cite this Reference:
S. Adeola Adesegun, O. Folorunso Samuel, B. Ojekale Anthony, B. Ogungbe Folasade and S. Kayode Mary, . Essential Oil of Syzygium samarangense; A Potent Antimicrobial and Inhibitor of Partially Purified and Characterized Extracellular Protease of Escherichia coli 25922. British Journal of Pharmacology and Toxicology, (6): 215-221.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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