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Abstract
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Article Information:
Toxicity Evaluation of the Liver and in vitro Metabolism in Wistar Rat on Exposure to N-Nitrosamine Precursors
Usunobun Usunomena, Josiah J. Sunday, Nwangwu Spencer, Uhunmwagho S. Esosa, Omage Kingsley and Maduagwu N. Emmanuel
Corresponding Author: Usunobun Usunomena
Submitted: 2011 May, 23
Accepted: 2011 July, 02
Published: 2011 August, 05 |
Abstract:
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The aim of this study is to evaluate liver toxicity on exposure to n-nitrosamine precursors as well
as the effect of ultraviolet light on n-nitrosamines and its precursors. Toxicological evaluation of the liver
following single dose treatment of wistar rat with 8.2125 mg NaNO2/adult rat and in rats given a combined dose
of 50 mgDMA-HCl and 8.2125 mg NaNO2/adult rat showed a steady elevation of the liver function enzymes.
Histopathological analysis of the liver showed hepatic necrosis in the chemical induced rats. Following UV
exposure after in vitro incubation of rat liver microsomal plus soluble fraction with NaNO2 and NaNO2 plus
DMA-HCl, nitrite concentration in the NaNO2 incubation medium was 19.5 and 2.2 μg/mL before and after
UV exposure respectively while the nitrite concentration in the NaNO2 plus DMA-HCl incubation medium was
23.5 and 2.5 μg/mL, respectively. Nitrite loss was significant (p<0.05) between before and after UV exposure
in all groups. UV exposure, thus degraded the nitrosamine precursors, nitrite and DMA-HCl, thereby inhibiting
possible nitrosation. The high values of the activities of serum transaminases (AST and ALT), alkaline
phosphatases (ALP) and gamma-glutamyltransferses (γ-GT), relative to control values are indicative of severe
intrahepatic cell damage.
Key words: Dimethylamine hydrochloride, incubation, N-nitrosamine, Sodium nitrite, UV irradiation, ,
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Cite this Reference:
Usunobun Usunomena, Josiah J. Sunday, Nwangwu Spencer, Uhunmwagho S. Esosa, Omage Kingsley and Maduagwu N. Emmanuel, . Toxicity Evaluation of the Liver and in vitro Metabolism in Wistar Rat on Exposure to N-Nitrosamine Precursors. British Journal of Pharmacology and Toxicology, (3): 138-142.
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ISSN (Online): 2044-2467
ISSN (Print): 2044-2459 |
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