Home           Contact us           FAQs           
 
   Journal Page   |   Aims & Scope   |   Author Guideline   |   Editorial Board   |   Search
    Abstract
2011 (Vol. 2, Issue: 2)
Article Information:

The Effect of Hypoxia on G Protein Coupled (Opioid) Receptor Gene Expression in Cortical B50 Neurons in Culture

A.O. Ibegbu, I. Mullaney, L. Fyfe and D. McBean
Corresponding Author:  Augustine Ibegbu 

Key words:  G-protein coupled receptor, hypoxia, messenger RNA, opioid agonist, opioid (mu) receptor, reverse transcription-PCR,
Vol. 2 , (2): 75-83
Submitted Accepted Published
2011 January, 26 2011 February, 23 2011 April, 30
Abstract:

Hypoxia adversely affects cells and tissues, and neuronal cells in particular have been shown to be more susceptible to the injurious effects of hypoxia in which they may begin to die when oxygen supply is reduced or completely eliminated. Opioid receptor agonists have been shown to elicit several central nervous system effects, mediated via G protein-coupled receptors. The aim of this study was to study the effect of hypoxia on G protein coupled receptor gene expression using mu opioid receptor as a case study in cortical neuronal B50 cell lines in culture. The B50 cells were cultured in normoxia (21% O2; 5% CO2) and hypoxia (5% O2; 5% CO2), and were treated with opioid agonists to determine their effects on hypoxia-induced changes. Three opioid agonists {DAMGO(μ), DSLET(δ) and ICI--199,441(κ)}, were administered to the cells as treatment for 48 h after 48 h of initial culture for a total of 96 h of culture in hypoxic conditions at concentrations of 10, 50 and 100 μM. The levels of G-protein coupled receptor (mu opioid) mRNAs were assessed using RT-PCR. The results showed that hypoxia induced morphological changes in B50 cells in hypoxia while the mu opioid RT-PCR mRNA levels showed no appreciable changes in normal, hypoxic and treated cells. The results show that B50 neuronal cells are susceptible to damage and injurious effects of hypoxia, as are most brain cells and the opioid agonist treatments showed there were no changes in the level of mu opioid receptor gene expression due to hypoxia or agonist treatment in neuronal B50 cells in culture.
Abstract PDF HTML
  Cite this Reference:
A.O. Ibegbu, I. Mullaney, L. Fyfe and D. McBean, 2011. The Effect of Hypoxia on G Protein Coupled (Opioid) Receptor Gene Expression in Cortical B50 Neurons in Culture.  British Journal of Pharmacology and Toxicology, 2(2): 75-83.
    Advertise with us
 
ISSN (Online):  2044-2467
ISSN (Print):   2044-2459
Submit Manuscript
   Current Information
   Sales & Services
   Contact Information
  Executive Managing Editor
  Email: admin@maxwellsci.com
  Publishing Editor
  Email: support@maxwellsci.com
  Account Manager
  Email: faisalm@maxwellsci.com
  Journal Editor
  Email: admin@maxwellsci.com
  Press Department
  Email: press@maxwellsci.com
Home  |  Contact us  |  About us  |  Privacy Policy
Copyright © 2009. MAXWELL Science Publication, a division of MAXWELLl Scientific Organization. All rights reserved