This investigation was carried out to isolate and identify the predominant microflora in Roub samples collected from different parts of the Sudan. A total of 30 samples were collected from El-Obied, North Kordofan State; Nyala, South Darfur State and Abu Naama, The Blue Nile State, in sterile bottles and transported in a cool box at 4ºC for analysis. The samples were subjected to microbiological examination (total viable bacteria, Staphylococcus aureus, Salmonella sp., lactic acid bacteria and yeasts and moulds). The results showed the occurrence of coliform bacteria, S. aureus, lactic acid bacteria, yeasts and moulds in the samples, while Salmonella sp. was not detected. Coliform bacteria were not detected in samples collected from Abu Naama area, and detected in 30 and 20% of samples collected from El Obied and Nyala areas respectively. S. aureus was detected in 40, 60 and 20% of samples from El Obied, Nyala and Abu Naama, respectively while yeasts and moulds were detected in 100, 90 and 90% of samples from El Obied, Nyala and Abu Naama areas, respectively. Llactic acid bacteria w ere detected in all samples under study.