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Abstract
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Article Information:
Preliminary Analysis of gra SR for Vancomycin Resistance in Staphylococcus aureus Local Isolates
Dlnya A. Mohamad
Corresponding Author: Dlnya A. Mohamad
Submitted: March 03, 2012
Accepted: March 24, 2012
Published: April 30, 2012 |
Abstract:
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Methicillin-resistant Staphylococcus aureus has become a frequent cause of nosocomial infection.
Extensive burn injuries are particularly susceptible to infection. Extended hospitalization and antibiotic therapy
have been identified as additional risk factors for MRSA carriage and infection, since prolonged use of
antibiotics change the bacterial response to the surrounding medium and increase the mutation rate especially
to the targeted site and also to the bacterial signal transduction system leading to the occurrence of the new
strain showing various resistant mechanisms to the old as well as new antibiotic such as vancomysin. The aim
of this study was to analyze response regulator graR gene to determine if the mutation in the signal transduction
system lead to the variable susceptibility to vancomycin. Ten VRSA isolated from burned patients in the Burns
Unit at sulaimani emergency hospital in 2008. All the isolates were $- lactamase producers. The PCR based
identification technique used in this study proved to be rapid and accurate for the detection the genes regulate
the vancomysin availability (sensor kinase and response regulator gra SR). Comparison of gra R DNA sequence
and translated nucleotide sequence with N315 DNA and translated nucleotide sequences showed substitution
of T553 instead of C, C554 instead of G, G556 instead of T, C565 instead of T and G 568 instead of A in N315 DNA
sequence. This nucleotide substitution result in alteration of Gln548 instead of Asp and Asn717 instead of Ser
amino acid in N315. No mutation was detected in graS gene.
Key words: Burn, mrsa, two component signal, vrsa, , ,
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Abstract
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Cite this Reference:
Dlnya A. Mohamad, . Preliminary Analysis of gra SR for Vancomycin Resistance in Staphylococcus aureus Local Isolates. Asian Journal of Medical Sciences, (2): 85-88.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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