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Abstract
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Article Information:
Antimicrobial Activity of Marine Actinomycete, Nocardiopsis sp. VITSVK 5 (FJ973467)
V. Vimal, Benita Mercy Rajan and K. Kannabiran
Corresponding Author: Dr.K.Kannabiran
Submitted: 2009 July, 21
Accepted: 2009 August, 01
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Abstract:
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The aim of the present study was to isolate and to indentify the actinomycetes having antagonistic activity.
An actinomycetes strain isolated from marine sediment samples collected at the Puducherry coast of India,
showed antibacterial activity against selected microbial pathogens. The nutritional requirements and cultural
conditions for maximal growth and yield of secondary metabolites have been optimized under shake-flask
conditions. The growth and yield of secondary metabolites was maximal with the use of ISP 1 medium
supplemented with sea water, pH 7.4, and incubation temperature of 28/ C, salt tolerance of 2% and incubation
time of 4-7 days. Based on morphological, biochemical, physiological and phylogenetic characterization, the
strain was identified as Nocardiopsis sp. VITSVK5 (FJ973467). The petroleum ether extract (1000 :g/ml)
obtained from the isolate showed significant antibacterial activity against Gram negative bacteria- Escherichia
coli (20 mm), Pseudomonas aeruginosa (18 mm) and Klebsiella pneumonia (15 mm) and Gram positive
bacteria- Enterococcus faecalis (20 mm), Bacillus cereus (13 mm) and Staphylococcus aureus (6 mm) when
compared with streptomycin (25 :g/disc). The ethyl acetate extract (1000 :g/ml) showed antifungal activity
against Aspergillus fumigatus (23 mm), Aspergillus flavus (15 mm) and Aspergillus niger (12 mm) when
compared with amphotericin-B (25 :g/disc). The chloroform extract (1000 :g/ml) was very effective against
yeasts, Candida cruzi (18 mm), Candida tropicans (15 mm) and Candida albicans (14 mm) when compared
to streptomycin (25 :g/disc). In conclusion the isolated strain has broad spectrum of antagonistic activity
against Gram positive and Gram negative bacteria and Aspergillus sp.
Key words: Actinomycetes, antagonistic activity, antifungal activity, Aspergillus sp., Nocardiopsis sp. VITSVK5, ,
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Cite this Reference:
V. Vimal, Benita Mercy Rajan and K. Kannabiran, . Antimicrobial Activity of Marine Actinomycete, Nocardiopsis sp. VITSVK 5 (FJ973467). Asian Journal of Medical Sciences, (2): Page No: 57-63.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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