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Abstract
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Article Information:
The Effect of Crude Methanolic Leaf Extract of Bryophyllum pinnatum on Some Haematological Parameters in Wistar Rats
S.A. Ufelle, E.O. Ukaejiofo, E.E. Neboh, P.U. Achukwu, S. Ghasi, E.J. Ikekpeazu and I.C. Maduka
Corresponding Author: Silas Ufelle
Submitted: 2011 April, 06
Accepted: 2011 May, 13
Published: 2011 June, 20 |
Abstract:
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Bryophyllum pinnatum (B. pinnatum) is a perennial herb growing widely and used in folkloric
medicine. Much research work has not been carried out on its effect on hematological parameters. We therefore
decided to investigate the effect of crude methanolic leaf extract of B. pinnatum on some hematological
parameters in Wistar rats. Twenty (20) male Wistar rats aged 2 to 3 months obtained from the Animal House
of College of Medicine, University of Nigeria Enugu Campus were acclimatized for two weeks. They were
divided into five groups labeled A to E. Groups A to D were orally fed with graded doses of the crude leaf
extract (100, 200, 400, and 600 mg/kg body weight respectively) once daily for 28days in lower concentrations
compared to an oral LD 50 of 800 mg/kg body weight. Group E served as control without receiving the extract.
On Day 29 about 2.5 mL of blood sample were collected from each rat through the median canthus into
K3-EDTA anticoagulant containers for hemoglobin (Hb), Packed Cell Volume (PCV), Total White Blood Cell
(TWBC), and Platelet count. The results revealed significantly increased Hb in all the treated groups,
A=15.9±1.0, B = 16.8±1.0, C = 17.5±1.0 and D = 18.7±1.0 g/dL when compared with control E = 13.5±0.5
g/dL (p<0.05). The PCV of the treated groups were also significantly increased, A = 0.48±0.01, B = 0.51±0.01,
C = 0.53±0.01 and D = 0.56±0.01 L/L when compared with control E = 0.38±0.01 L/L (p<0.05). The TWBC
were significantly increased in all the treated groups (A = 6.2±1.0, B = 6.5±1.0, C = 7.1±1.0
and D = 7.7±1.0×109/L when compared with control E = 4.0±1.0×109/L. The platelet count were decreased in
all the treated groups but was significant only in group A = 135±13×109/L when compared with control
E = 225±20×109/L (p<0.05). The blood film examination revealed normocytic and normochromic red blood
cells. This result pattern suggests that crude methanolic leaf extract of B. pinnatum may have properties that
increase the Hb, PCV and TWBC, while decreasing the platelets, hence care should be taken while consuming
such extracts to avoid haematological disturbances such as thrombocytopaenia. Also, constant monitoring the
hematological parameters should be ensured in subjects taking such medicinal herbs for whatever purposes.
Key words: Bryophyllum pinnatum, hematological parameters, methanolic leaf extract, , , ,
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Cite this Reference:
S.A. Ufelle, E.O. Ukaejiofo, E.E. Neboh, P.U. Achukwu, S. Ghasi, E.J. Ikekpeazu and I.C. Maduka, . The Effect of Crude Methanolic Leaf Extract of Bryophyllum pinnatum on Some Haematological Parameters in Wistar Rats. Asian Journal of Medical Sciences, (3): 121-124.
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ISSN (Online): 2040-8773
ISSN (Print): 2040-8765 |
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