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2010 (Vol. 2, Issue: 5)
Article Information:

Temperature Adaptation Study on Probiotic Bacillus subtilis SK09 Based on its Extracellular Proteins

G. Sreekumar and K. Soundarajan
Corresponding Author:  G. Sreekumar 

Key words:  Amylase, β - galactosidase, thermal adaptation, thermal stress, probiotic, ,
Vol. 2 , (5): Page No: 246-249
Submitted Accepted Published
2010 July, 01 2010 July, 27 2010 September, 25
Abstract:

The assessment of the thermal adaptation characteristics of a probiotic organism is crucial since the probiotic bacteria are sensitive to thermal stress created by the gut environment. Probiotic Bacillus subtilis SK09 was subjected to varying temperatures growth conditions and checked for its thermal adaptation. Experiments were carried out with cultures grown in Nutrient broth at three different temperatures namely 25, 35 and 40C. After 24 h the extracellular secretions present in the cell free supernatant were analyzed for amylase and $-galactosidase activity as well the net protein content. Bacillus subtilis SK09 showed a prominent growth and protein profile in 35C with maximum enzyme activity and total protein production compared to other two conditions. At 40C the strain exhibited a 30 kDa protein which might have been induced due to thermal stress.
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  Cite this Reference:
G. Sreekumar and K. Soundarajan, 2010. Temperature Adaptation Study on Probiotic Bacillus subtilis SK09 Based on its Extracellular Proteins.  Advance Journal of Food Science and Technology, 2(5): Page No: 246-249.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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