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     Advance Journal of Food Science and Technology

    Abstract
2015(Vol.8, Issue:9)
Article Information:

Detection of Staphylococcus aureus in Milk Using Real-time Fluorescence Loop-mediated Isothermal Amplification

Ying Yu, Xiaoyan Ma and Wei Zhang
Corresponding Author:  Wei Zhang 
Submitted: ‎January ‎8, ‎2015
Accepted: ‎February ‎5, ‎2015
Published: July 05, 2015
Abstract:
Staphylococcus aureus is a kind of worldwide food-borne pathogen. Recently, S. aureus has gained considerable attention because of the increasing alimentary toxicosis incidence. In this study, a Real-Time fluorescence Loop-Mediated isothermal Amplification (RT-LAMP) was developed to detect S. aureus rapidly. The heat-stable nuclease (nuc) gene of S. aureus, the target sequence, was selected to design four special primers. A rapid detection method for S. aureus was initially established under optimum reaction conditions. The assay, performed for 40 min at 61°C, did not show cross reactivity with other bacterial species. The specificity and sensitivity of RT-LAMP for detecting S. aureus were 100% and 8.0 CFU/mL, respectively. Results indicated that RT-LAMP was a potential field-usable molecular tool for detecting S. aureus This method can be an alternative to conventional LAMP in clinical applications and operational programs.

Key words:  Detection, nuclease gene, rapid, sensitivity, specificity, ,
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Cite this Reference:
Ying Yu, Xiaoyan Ma and Wei Zhang, . Detection of Staphylococcus aureus in Milk Using Real-time Fluorescence Loop-mediated Isothermal Amplification. Advance Journal of Food S`cience and Technology, (9): 678-684.
ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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