Abstract
|
Article Information:
Detection of Staphylococcus aureus in Milk Using Real-time Fluorescence Loop-mediated Isothermal Amplification
Ying Yu, Xiaoyan Ma and Wei Zhang
Corresponding Author: Wei Zhang
Submitted: January 8, 2015
Accepted: February 5, 2015
Published: July 05, 2015 |
Abstract:
|
Staphylococcus aureus is a kind of worldwide food-borne pathogen. Recently, S. aureus has gained considerable attention because of the increasing alimentary toxicosis incidence. In this study, a Real-Time fluorescence Loop-Mediated isothermal Amplification (RT-LAMP) was developed to detect S. aureus rapidly. The heat-stable nuclease (nuc) gene of S. aureus, the target sequence, was selected to design four special primers. A rapid detection method for S. aureus was initially established under optimum reaction conditions. The assay, performed for 40 min at 61°C, did not show cross reactivity with other bacterial species. The specificity and sensitivity of RT-LAMP for detecting S. aureus were 100% and 8.0 CFU/mL, respectively. Results indicated that RT-LAMP was a potential field-usable molecular tool for detecting S. aureus This method can be an alternative to conventional LAMP in clinical applications and operational programs.
Key words: Detection, nuclease gene, rapid, sensitivity, specificity, ,
|
Abstract
|
PDF
|
HTML |
|
Cite this Reference:
Ying Yu, Xiaoyan Ma and Wei Zhang, . Detection of Staphylococcus aureus in Milk Using Real-time Fluorescence Loop-mediated Isothermal Amplification. Advance Journal of Food S`cience and Technology, (9): 678-684.
|
|
|
|
|
ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
|
Information |
|
|
|
Sales & Services |
|
|
|