Abstract
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Article Information:
Application of Plackett-Burman Design in Screening of Carbohydrate, Prebiotics/Alcohols and Protein/Amino Acid for Cryoprotectants of Streptococcus thermophilus
He Chen, Yixin Hui, Hongli Chen, Yanyan Wu and Guowei Shu
Corresponding Author: Guowei Shu
Submitted: August 19, 2014
Accepted: September 24, 2014
Published: February 15, 2015 |
Abstract:
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The carbohydrate, prebiotics/alcohols as well as protein/amino acid have shown important influence on the cryoprotectants for Streptococcus thermophilus, this study aimed to screen the main effective factors for cryoprotectants of Streptococcus thermophiles using Plackett-Burman design. Viable counts with cryoprotectants were studied containing eight carbohydrate (glucose, fructose, sucrose, lactose, galactose maltose, trehalose, soluble starch), eleven prebiotics/alcohols (xylooligosaccharide, fructo-oligosaccharide, galactooligosaccharide, isomaltooligosaccharide, stachyose, synanthrin, dextran sulfate, glycerin, sorbitol, mannitol, inositol) and eight protein/amino acid (peptone, yeast powder, casein hydrolysate, glutamic acid, methionine, cysteine, alanine, Vc). The results indicated that sucrose and soluble starch, dextran sulfate, casein hydrolysate among the carbohydrate, prebiotics/alcohols and protein/amino acid affected the cryoprotectants for Streptococcus thermophilus markly, which should be appropriately increased in the subsequent experiments.
Key words: Carbohydrate, cryoprotectants, prebiotics/alcohols,, protein/amino acid, Streptococcus thermophiles, ,
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Cite this Reference:
He Chen, Yixin Hui, Hongli Chen, Yanyan Wu and Guowei Shu, . Application of Plackett-Burman Design in Screening of Carbohydrate, Prebiotics/Alcohols and Protein/Amino Acid for Cryoprotectants of Streptococcus thermophilus. Advance Journal of Food Science and Technology, (5): 314-319.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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