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Abstract
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Article Information:
Rapid Simultaneous Analysis for Three Sugars Fraction in Enzymatic Degradation of Stachyose
Suhong Li, Xue Bai, Mosi Dong, 2Nan Li and Tuoping Li
Corresponding Author: Suhong Li
Submitted: February 06, 2014
Accepted: April 09, 2014
Published: August 10, 2014 |
Abstract:
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The objective of the present study was to develop an effective method of High Performance Liquid Chromatography-Refractive Index Detector (HPLC-RID) for the simultaneous quantification of stachyose, raffinose and galactose in enzymatic degradation of stachyose. The three sugars were separated on an Agilent zorbax carbohydrate column with acetonitrile water (75:25 v/v). The calibration curves of the three standards exhibited good linearity (R2 >0.9989). The Limits of Detection (LOD) values of the stachyose, raffinose and galactose were 0.137, 0.301 and 0.386 μg/mL, respectively and their Limits of Quantification (LOQ) values were 0.452, 0.993 and 1.25 &mug/mL, respectively. The Relative Standard Deviations (RSDs) of the data of the intra- and inter-day experiments were less than 2.68, 2.42 and 3.70%, respectively. The results of the recovery test were 97.71-102.61% with an RSD value of 0.92-2.41%. In conclusion, the results of validation showed that the proposed HPLC-RID method was useful for the quantification of the three sugars in enzymatic degradation of stachyose and similar reaction.
Key words: Galactose, HPLC-RID, raffinose, stachyose, , ,
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Abstract
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Cite this Reference:
Suhong Li, Xue Bai, Mosi Dong, 2Nan Li and Tuoping Li , . Rapid Simultaneous Analysis for Three Sugars Fraction in Enzymatic Degradation of Stachyose. Advance Journal of Food Science and Technology, (8): 951-955.
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ISSN (Online): 2042-4876
ISSN (Print): 2042-4868 |
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