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    Abstract
2014 (Vol. 6, Issue: 5)
Article Information:

Optimization of Combined Pulsed Electric Fields and Mild Temperature Processing Conditions for Red Apple Juice Polyphenol Oxidase and Peroxidase Inactivation

Wendy Katiyo, Ruijin Yang, Wei Zhao, Xiao Hua and Mohammed Abdalbasit Ahmed Gasmalla
Corresponding Author:  Ruijin Yang 

Key words:  Color, combined PEF and mild temperature, peroxidase, polyphenol oxidase, red apple juice, response surface methodology,
Vol. 6 , (5): 638-646
Submitted Accepted Published
January 24, 2014 February 10, 2014 May 10, 2014
Abstract:

The effect on Polyphenol Oxidase (PPO) and Peroxidase (POD) enzyme activity in red apple juice was evaluated after combined Pulsed Electric Fields (PEF) and mild temperature processing using a response surface methodology. Changes in color were also analyzed and compared with thermally treated and unpasteurized juices. The studied factors were electric field strength (10-30 kV/cm), treatment time (200-1000 s) and temperature (20-60C). A significant second-order response function covering the whole range of experimental conditions was obtained for each enzyme. Treatments conducted at 30 kV/cm, 1000 s and 60C led to red apple juice with the lowest residual enzyme activity (0.04 and 0.16 for PPO and POD, respectively). Overall change in color was significantly lower (p<0.05), in comparison with severe thermal treatments. It was feasible to achieve comparable enzyme inactivation and better preserve natural juice color by this hurdle technique.
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  Cite this Reference:
Wendy Katiyo, Ruijin Yang, Wei Zhao, Xiao Hua and Mohammed Abdalbasit Ahmed Gasmalla, 2014. Optimization of Combined Pulsed Electric Fields and Mild Temperature Processing Conditions for Red Apple Juice Polyphenol Oxidase and Peroxidase Inactivation.  Advance Journal of Food Science and Technology, 6(5): 638-646.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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