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2014 (Vol. 6, Issue: 5)
Article Information:

Application of TLC in the Screening of Acarbose-producing Actinomycetes

Fei Ren, Long Chen and Qunyi Tong
Corresponding Author:  Qunyi Tong 

Key words:  Acarbose, actinomycetes, diabetes mellitus, screening, TLC, ,
Vol. 6 , (5): 629-633
Submitted Accepted Published
January 20, 2014 January 30, 2014 May 10, 2014

Acarbose is widely used in medicine, such as the treatment of diabetes and obesity. A simple Thin Layer Chromatography (TLC)-scanning technique was developed for the rapid and accurate analysis of acarbose in a large number of fermentation broths of actinomycetes to screen for acarbose producer. The linearity of the acarbose in this way was good within the range from 2 to 10 μg (r2 = 0.9997). This technique didnít need expensive instrument and complex procedure for the detection of acarbose in the fermentation broths. An actinomycete with acarbose yield of 1.83 mg/mL was obtained. The results demonstrated that TLC-scanning was a cheap and simple technique for the accurate screening of acarbose-producing actinomycetes.
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  Cite this Reference:
Fei Ren, Long Chen and Qunyi Tong, 2014. Application of TLC in the Screening of Acarbose-producing Actinomycetes.  Advance Journal of Food Science and Technology, 6(5): 629-633.
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ISSN (Online):  2042-4876
ISSN (Print):   2042-4868
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